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Pairing of VH gene families with the lambda 1 light chain: evidence for a non-stochastic association.

Publication ,  Journal Article
Yurovsky, VV; Kelsoe, G
Published in: Eur J Immunol
August 1993

The frequencies at which four VH gene families pair with the lambda 1 light (L) chain were determined by sequential hybridization of VH- and lambda 1-specific DNA probes to mitogen-induced colonies of B cells. Analysis of pair frequencies indicates that the repertoire of lambda L chain antibodies is generated by the stochastic pairing of smaller 3'-to-mid-locus VH gene families (X-24, S107, Q52). However, the large 5' VH J558 family appeared to associate with the lambda 1 L chain non-stochastically; the frequency of VHJ558/lambda 1+ colonies among all lambda 1+ colonies was significantly lower than the frequency of J558 expression among all (C mu+) B cell colonies. This difference suggests that selection, either intrinsic at the level of rearrangement or heavy and L chain pairing, or extrinsic following surface immunoglobulin expression, may operate to shape the lambda antibody repertoire prior to the introduction of exogenous antigen.

Duke Scholars

Published In

Eur J Immunol

DOI

ISSN

0014-2980

Publication Date

August 1993

Volume

23

Issue

8

Start / End Page

1975 / 1979

Location

Germany

Related Subject Headings

  • Mice, Inbred C57BL
  • Mice
  • Lymphocyte Activation
  • Immunology
  • Immunoglobulin lambda-Chains
  • Immunoglobulin Variable Region
  • Immunoglobulin Heavy Chains
  • Genes, Immunoglobulin
  • Cell Separation
  • Animals
 

Citation

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Yurovsky, V. V., & Kelsoe, G. (1993). Pairing of VH gene families with the lambda 1 light chain: evidence for a non-stochastic association. Eur J Immunol, 23(8), 1975–1979. https://doi.org/10.1002/eji.1830230837
Yurovsky, V. V., and G. Kelsoe. “Pairing of VH gene families with the lambda 1 light chain: evidence for a non-stochastic association.Eur J Immunol 23, no. 8 (August 1993): 1975–79. https://doi.org/10.1002/eji.1830230837.
Yurovsky, V. V., and G. Kelsoe. “Pairing of VH gene families with the lambda 1 light chain: evidence for a non-stochastic association.Eur J Immunol, vol. 23, no. 8, Aug. 1993, pp. 1975–79. Pubmed, doi:10.1002/eji.1830230837.
Journal cover image

Published In

Eur J Immunol

DOI

ISSN

0014-2980

Publication Date

August 1993

Volume

23

Issue

8

Start / End Page

1975 / 1979

Location

Germany

Related Subject Headings

  • Mice, Inbred C57BL
  • Mice
  • Lymphocyte Activation
  • Immunology
  • Immunoglobulin lambda-Chains
  • Immunoglobulin Variable Region
  • Immunoglobulin Heavy Chains
  • Genes, Immunoglobulin
  • Cell Separation
  • Animals