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Identification of the endogenous smooth muscle myosin phosphatase-associated kinase.

Publication ,  Journal Article
MacDonald, JA; Borman, MA; Murányi, A; Somlyo, AV; Hartshorne, DJ; Haystead, TA
Published in: Proc Natl Acad Sci U S A
February 27, 2001

Ca(2+) sensitization of smooth muscle contraction involves inhibition of myosin light chain phosphatase (SMPP-1M) and enhanced myosin light chain phosphorylation. Inhibition of SMPP-1M is modulated through phosphorylation of the myosin targeting subunit (MYPT1) by either Rho-associated kinase (ROK) or an unknown SMPP-1M-associated kinase. Activated ROK is predominantly membrane-associated and its putative substrate, SMPP-1M, is mainly myofibrillar-associated. This raises a conundrum about the mechanism of interaction between these enzymes. We present ZIP-like kinase, identified by "mixed-peptide" Edman sequencing after affinity purification, as the previously unidentified SMPP-1M-associated kinase. ZIP-like kinase was shown to associate with MYPT1 and phosphorylate the inhibitory site in intact smooth muscle. Phosphorylation of ZIP-like kinase was associated with an increase in kinase activity during carbachol stimulation, suggesting that the enzyme may be a terminal member of a Ca(2+) sensitizing kinase cascade.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

February 27, 2001

Volume

98

Issue

5

Start / End Page

2419 / 2424

Location

United States

Related Subject Headings

  • Rabbits
  • Protein Serine-Threonine Kinases
  • Precipitin Tests
  • Phosphorylation
  • Muscle, Smooth
  • Molecular Sequence Data
  • Electrophoresis, Polyacrylamide Gel
  • Death-Associated Protein Kinases
  • Chromatography, Ion Exchange
  • Cattle
 

Citation

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MacDonald, J. A., Borman, M. A., Murányi, A., Somlyo, A. V., Hartshorne, D. J., & Haystead, T. A. (2001). Identification of the endogenous smooth muscle myosin phosphatase-associated kinase. Proc Natl Acad Sci U S A, 98(5), 2419–2424. https://doi.org/10.1073/pnas.041331498
MacDonald, J. A., M. A. Borman, A. Murányi, A. V. Somlyo, D. J. Hartshorne, and T. A. Haystead. “Identification of the endogenous smooth muscle myosin phosphatase-associated kinase.Proc Natl Acad Sci U S A 98, no. 5 (February 27, 2001): 2419–24. https://doi.org/10.1073/pnas.041331498.
MacDonald JA, Borman MA, Murányi A, Somlyo AV, Hartshorne DJ, Haystead TA. Identification of the endogenous smooth muscle myosin phosphatase-associated kinase. Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2419–24.
MacDonald, J. A., et al. “Identification of the endogenous smooth muscle myosin phosphatase-associated kinase.Proc Natl Acad Sci U S A, vol. 98, no. 5, Feb. 2001, pp. 2419–24. Pubmed, doi:10.1073/pnas.041331498.
MacDonald JA, Borman MA, Murányi A, Somlyo AV, Hartshorne DJ, Haystead TA. Identification of the endogenous smooth muscle myosin phosphatase-associated kinase. Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2419–2424.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

February 27, 2001

Volume

98

Issue

5

Start / End Page

2419 / 2424

Location

United States

Related Subject Headings

  • Rabbits
  • Protein Serine-Threonine Kinases
  • Precipitin Tests
  • Phosphorylation
  • Muscle, Smooth
  • Molecular Sequence Data
  • Electrophoresis, Polyacrylamide Gel
  • Death-Associated Protein Kinases
  • Chromatography, Ion Exchange
  • Cattle