Skip to main content
Journal cover image

Phosphorylation of the rRNA transcription factor upstream binding factor promotes its association with TATA binding protein.

Publication ,  Journal Article
Kihm, AJ; Hershey, JC; Haystead, TA; Madsen, CS; Owens, GK
Published in: Proc Natl Acad Sci U S A
December 8, 1998

rRNA synthesis by RNA polymerase I requires both the promoter selectivity factor 1, which is composed of TATA binding protein (TBP) and three TBP-associated factors, and the activator upstream binding factor (UBF). Whereas there is strong evidence implicating a role for phosphorylation of UBF in the control of growth-induced increases in rRNA transcription, the mechanism of this effect is not known. Results of immunoprecipitation studies with TBP antibodies showed increased recovery of phosphorylated UBF from growth-stimulated smooth muscle cells. Moreover, using an immobilized protein-binding assay, we found that phosphorylation of UBF in vivo in response to stimulation with different growth factors or in vitro with smooth muscle cell nuclear extract increased its binding to TBP. Finally, we demonstrated that UBF-TBP binding depended on the C-terminal 'acidic tail' of UBF that was hyperphosphorylated at multiple serine sites after growth factor stimulation. Results of these studies suggest that phosphorylation of UBF and subsequent binding to TBP represent a key regulatory step in control of growth-induced increases in rRNA synthesis.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

December 8, 1998

Volume

95

Issue

25

Start / End Page

14816 / 14820

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • TATA Box
  • Signal Transduction
  • Rats
  • RNA, Ribosomal
  • Protein Binding
  • Pol1 Transcription Initiation Complex Proteins
  • Phosphorylation
  • Muscle, Smooth, Vascular
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kihm, A. J., Hershey, J. C., Haystead, T. A., Madsen, C. S., & Owens, G. K. (1998). Phosphorylation of the rRNA transcription factor upstream binding factor promotes its association with TATA binding protein. Proc Natl Acad Sci U S A, 95(25), 14816–14820. https://doi.org/10.1073/pnas.95.25.14816
Kihm, A. J., J. C. Hershey, T. A. Haystead, C. S. Madsen, and G. K. Owens. “Phosphorylation of the rRNA transcription factor upstream binding factor promotes its association with TATA binding protein.Proc Natl Acad Sci U S A 95, no. 25 (December 8, 1998): 14816–20. https://doi.org/10.1073/pnas.95.25.14816.
Kihm AJ, Hershey JC, Haystead TA, Madsen CS, Owens GK. Phosphorylation of the rRNA transcription factor upstream binding factor promotes its association with TATA binding protein. Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14816–20.
Kihm, A. J., et al. “Phosphorylation of the rRNA transcription factor upstream binding factor promotes its association with TATA binding protein.Proc Natl Acad Sci U S A, vol. 95, no. 25, Dec. 1998, pp. 14816–20. Pubmed, doi:10.1073/pnas.95.25.14816.
Kihm AJ, Hershey JC, Haystead TA, Madsen CS, Owens GK. Phosphorylation of the rRNA transcription factor upstream binding factor promotes its association with TATA binding protein. Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14816–14820.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

December 8, 1998

Volume

95

Issue

25

Start / End Page

14816 / 14820

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • TATA Box
  • Signal Transduction
  • Rats
  • RNA, Ribosomal
  • Protein Binding
  • Pol1 Transcription Initiation Complex Proteins
  • Phosphorylation
  • Muscle, Smooth, Vascular