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Metabolic regulation of oocyte cell death through the CaMKII-mediated phosphorylation of caspase-2.

Publication ,  Journal Article
Nutt, LK; Margolis, SS; Jensen, M; Herman, CE; Dunphy, WG; Rathmell, JC; Kornbluth, S
Published in: Cell
October 2005

Vertebrate female reproduction is limited by the oocyte stockpiles acquired during embryonic development. These are gradually depleted over the organism's lifetime through the process of apoptosis. The timer that triggers this cell death is yet to be identified. We used the Xenopus egg/oocyte system to examine the hypothesis that nutrient stores can regulate oocyte viability. We show that pentose-phosphate-pathway generation of NADPH is critical for oocyte survival and that the target of this regulation is caspase-2, previously shown to be required for oocyte death in mice. Pentose-phosphate-pathway-mediated inhibition of cell death was due to the inhibitory phosphorylation of caspase-2 by calcium/calmodulin-dependent protein kinase II (CaMKII). These data suggest that exhaustion of oocyte nutrients, resulting in an inability to generate NADPH, may contribute to ooctye apoptosis. These data also provide unexpected links between oocyte metabolism, CaMKII, and caspase-2.

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Published In

Cell

DOI

EISSN

1097-4172

ISSN

0092-8674

Publication Date

October 2005

Volume

123

Issue

1

Start / End Page

89 / 103

Related Subject Headings

  • Xenopus laevis
  • Signal Transduction
  • Reproduction
  • Phosphorylation
  • Pentose Phosphate Pathway
  • Oogenesis
  • Oocytes
  • NADP
  • Glucose
  • Female
 

Citation

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ICMJE
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Nutt, L. K., Margolis, S. S., Jensen, M., Herman, C. E., Dunphy, W. G., Rathmell, J. C., & Kornbluth, S. (2005). Metabolic regulation of oocyte cell death through the CaMKII-mediated phosphorylation of caspase-2. Cell, 123(1), 89–103. https://doi.org/10.1016/j.cell.2005.07.032
Nutt, Leta K., Seth S. Margolis, Mette Jensen, Catherine E. Herman, William G. Dunphy, Jeffrey C. Rathmell, and Sally Kornbluth. “Metabolic regulation of oocyte cell death through the CaMKII-mediated phosphorylation of caspase-2.Cell 123, no. 1 (October 2005): 89–103. https://doi.org/10.1016/j.cell.2005.07.032.
Nutt LK, Margolis SS, Jensen M, Herman CE, Dunphy WG, Rathmell JC, et al. Metabolic regulation of oocyte cell death through the CaMKII-mediated phosphorylation of caspase-2. Cell. 2005 Oct;123(1):89–103.
Nutt, Leta K., et al. “Metabolic regulation of oocyte cell death through the CaMKII-mediated phosphorylation of caspase-2.Cell, vol. 123, no. 1, Oct. 2005, pp. 89–103. Epmc, doi:10.1016/j.cell.2005.07.032.
Nutt LK, Margolis SS, Jensen M, Herman CE, Dunphy WG, Rathmell JC, Kornbluth S. Metabolic regulation of oocyte cell death through the CaMKII-mediated phosphorylation of caspase-2. Cell. 2005 Oct;123(1):89–103.
Journal cover image

Published In

Cell

DOI

EISSN

1097-4172

ISSN

0092-8674

Publication Date

October 2005

Volume

123

Issue

1

Start / End Page

89 / 103

Related Subject Headings

  • Xenopus laevis
  • Signal Transduction
  • Reproduction
  • Phosphorylation
  • Pentose Phosphate Pathway
  • Oogenesis
  • Oocytes
  • NADP
  • Glucose
  • Female