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The zinc finger cluster domain of RanBP2 is a specific docking site for the nuclear export factor, exportin-1.

Publication ,  Journal Article
Singh, BB; Patel, HH; Roepman, R; Schick, D; Ferreira, PA
Published in: J Biol Chem
December 24, 1999

The Ran-binding protein 2 (RanBP2) is a large scaffold cyclophilin-related protein expressed in photoreceptor cells. Red/green opsin, Ran-GTPase, and the 19 S regulatory complex of the proteasome associate with specific RanBP2 structural modules. Some of these play a role in chaperoning the functional expression of opsin. RanBP2 localization at cytoplasmic fibrils emanating from the nuclear pore complex and interaction with the Ran-GTPase support also its role in nucleocytoplasmic transport processes. The degenerate nucleoporin repeat motifs FXFG, GLFG, and XXFG have been proposed to mediate the movement of nucleocytoplasmic transport factors. In particular, RanBP2 has been implicated in nuclear import processes. Here, we show the zinc fingers of RanBP2 associate with high specificity to the nuclear export factor, exportin-1 (CRM1). The bovine RanBP2 transcript contained only five of the eight zinc fingers reported in the human counterpart and are sufficient for exportin-1 association with RanBP2. In contrast to Ran interaction with RanBP2-exportin-1 complex, exportin-1 binding to the zinc finger cluster domain of RanBP2 is insensitive to leptomycin B and nucleotide-bound state of Ran-GTPase. Our results indicate that the zinc finger-rich domain of RanBP2 constitutes a docking site for exportin-1 during nuclear export. Thus, RanBP2 emerges as a key component of the nuclear export pathway.

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Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

December 24, 1999

Volume

274

Issue

52

Start / End Page

37370 / 37378

Location

United States

Related Subject Headings

  • Zinc Fingers
  • Zinc
  • Retinaldehyde
  • Receptors, Cytoplasmic and Nuclear
  • Nuclear Proteins
  • Nuclear Pore Complex Proteins
  • Molecular Sequence Data
  • Molecular Chaperones
  • Mice
  • Karyopherins
 

Citation

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Singh, B. B., Patel, H. H., Roepman, R., Schick, D., & Ferreira, P. A. (1999). The zinc finger cluster domain of RanBP2 is a specific docking site for the nuclear export factor, exportin-1. J Biol Chem, 274(52), 37370–37378. https://doi.org/10.1074/jbc.274.52.37370
Singh, B. B., H. H. Patel, R. Roepman, D. Schick, and P. A. Ferreira. “The zinc finger cluster domain of RanBP2 is a specific docking site for the nuclear export factor, exportin-1.J Biol Chem 274, no. 52 (December 24, 1999): 37370–78. https://doi.org/10.1074/jbc.274.52.37370.
Singh BB, Patel HH, Roepman R, Schick D, Ferreira PA. The zinc finger cluster domain of RanBP2 is a specific docking site for the nuclear export factor, exportin-1. J Biol Chem. 1999 Dec 24;274(52):37370–8.
Singh, B. B., et al. “The zinc finger cluster domain of RanBP2 is a specific docking site for the nuclear export factor, exportin-1.J Biol Chem, vol. 274, no. 52, Dec. 1999, pp. 37370–78. Pubmed, doi:10.1074/jbc.274.52.37370.
Singh BB, Patel HH, Roepman R, Schick D, Ferreira PA. The zinc finger cluster domain of RanBP2 is a specific docking site for the nuclear export factor, exportin-1. J Biol Chem. 1999 Dec 24;274(52):37370–37378.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

December 24, 1999

Volume

274

Issue

52

Start / End Page

37370 / 37378

Location

United States

Related Subject Headings

  • Zinc Fingers
  • Zinc
  • Retinaldehyde
  • Receptors, Cytoplasmic and Nuclear
  • Nuclear Proteins
  • Nuclear Pore Complex Proteins
  • Molecular Sequence Data
  • Molecular Chaperones
  • Mice
  • Karyopherins