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Alterations in the gamma-aminobutyric acid-gated chloride channel following transient forebrain ischemia in the gerbil.

Publication ,  Journal Article
Mileson, BE; Ehrmann, ML; Schwartz, RD
Published in: J Neurochem
February 1992

The role of inhibitory neurotransmission in selective neuronal degeneration after transient forebrain ischemia was studied by binding of t-[35S]butylbicyclophosphorothionate ([35S]TBPS) to the gamma-aminobutyric acid (GABA)-gated chloride channel and measurement of GABAA receptor function in Mongolian gerbil brain. [35S]TBPS binding to the hippocampus, striatum, and cortex quantified by autoradiography and muscimol-stimulated 36Cl- uptake in synaptoneurosomes of the same regions were examined 1, 4, and 29 days after a 5-min bilateral carotid occlusion. [35S]TBPS binding was decreased in the pyramidal cell dendritic layers, stratum oriens, and stratum lacunosum-moleculare of the CA1 hippocampus, 4 and 29 days after occlusion, and in the stratum radiatum 29 days after occlusion. [35S]TBPS binding sites in the lateral striatum decreased 47% 4 days after occlusion. At the same time, there was a corresponding decrease in muscimol-stimulated 36Cl- uptake in the striatal synaptoneurosomes. Muscimol-stimulated 36Cl- uptake in the hippocampus decreased slightly 4 days after occlusion and more so after 29 days, although these decreases were not significant. No changes were observed in somatosensory cortex at any time point. These data suggest that a portion of GABAA receptors in areas sensitive to ischemic insult are associated with degenerating neurons, whereas other GABAA) receptors are spared.

Duke Scholars

Published In

J Neurochem

DOI

ISSN

0022-3042

Publication Date

February 1992

Volume

58

Issue

2

Start / End Page

600 / 607

Location

England

Related Subject Headings

  • gamma-Aminobutyric Acid
  • Neurology & Neurosurgery
  • Muscimol
  • Motor Activity
  • Membrane Proteins
  • Male
  • Ischemic Attack, Transient
  • Ion Channel Gating
  • Gerbillinae
  • Chlorides
 

Citation

APA
Chicago
ICMJE
MLA
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Mileson, B. E., Ehrmann, M. L., & Schwartz, R. D. (1992). Alterations in the gamma-aminobutyric acid-gated chloride channel following transient forebrain ischemia in the gerbil. J Neurochem, 58(2), 600–607. https://doi.org/10.1111/j.1471-4159.1992.tb09761.x
Mileson, B. E., M. L. Ehrmann, and R. D. Schwartz. “Alterations in the gamma-aminobutyric acid-gated chloride channel following transient forebrain ischemia in the gerbil.J Neurochem 58, no. 2 (February 1992): 600–607. https://doi.org/10.1111/j.1471-4159.1992.tb09761.x.
Mileson, B. E., et al. “Alterations in the gamma-aminobutyric acid-gated chloride channel following transient forebrain ischemia in the gerbil.J Neurochem, vol. 58, no. 2, Feb. 1992, pp. 600–07. Pubmed, doi:10.1111/j.1471-4159.1992.tb09761.x.
Journal cover image

Published In

J Neurochem

DOI

ISSN

0022-3042

Publication Date

February 1992

Volume

58

Issue

2

Start / End Page

600 / 607

Location

England

Related Subject Headings

  • gamma-Aminobutyric Acid
  • Neurology & Neurosurgery
  • Muscimol
  • Motor Activity
  • Membrane Proteins
  • Male
  • Ischemic Attack, Transient
  • Ion Channel Gating
  • Gerbillinae
  • Chlorides