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Autoradiographic distribution of high affinity muscarinic and nicotinic cholinergic receptors labeled with [3H]acetylcholine in rat brain.

Publication ,  Journal Article
Schwartz, RD
Published in: Life Sci
June 9, 1986

The relative distribution of muscarinic and nicotinic cholinergic receptors labeled with [3H]acetylcholine was determined using autoradiography. [3H]Acetylcholine binding to high affinity muscarinic receptors was similar to what has been described for an M-2 distribution: highest levels of binding occurred in the pontine and brainstem nuclei, anterior pretectal area and anteroventral thalamic nucleus, while lower levels occurred in the caudate-putamen, accumbens nucleus and primary olfactory cortex. Nicotinic receptors were labeled with [3H]acetylcholine to the greatest extent in the interpeduncular nucleus, several thalamic nuclei, medial habenula, presubiculum and superior colliculus, and to the least extent in the hippocampus and inferior colliculus. By using autoradiography to localize cholinergic binding sites throughout the brain it was observed that the distributions of high affinity muscarinic and nicotinic sites labeled with the endogenous ligand, [3H]acetylcholine are different from each other and are different from distributions of muscarinic and nicotinic sites labeled with muscarinic and nicotinic antagonists.

Duke Scholars

Published In

Life Sci

DOI

ISSN

0024-3205

Publication Date

June 9, 1986

Volume

38

Issue

23

Start / End Page

2111 / 2119

Location

Netherlands

Related Subject Headings

  • Tissue Distribution
  • Receptors, Nicotinic
  • Receptors, Muscarinic
  • Rats, Inbred Strains
  • Rats
  • Pharmacology & Pharmacy
  • Male
  • Brain Chemistry
  • Autoradiography
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Journal cover image

Published In

Life Sci

DOI

ISSN

0024-3205

Publication Date

June 9, 1986

Volume

38

Issue

23

Start / End Page

2111 / 2119

Location

Netherlands

Related Subject Headings

  • Tissue Distribution
  • Receptors, Nicotinic
  • Receptors, Muscarinic
  • Rats, Inbred Strains
  • Rats
  • Pharmacology & Pharmacy
  • Male
  • Brain Chemistry
  • Autoradiography
  • Animals