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Mouse spinal cord compression injury is ameliorated by intrathecal cationic manganese(III) porphyrin catalytic antioxidant therapy.

Publication ,  Journal Article
Sheng, H; Spasojevic, I; Warner, DS; Batinic-Haberle, I
Published in: Neurosci Lett
August 12, 2004

This study evaluated the effects of the cationic manganese(III) tetrakis(N,N'-diethylimidazolium-2-yl)porphyrin catalytic antioxidant Mn(III)TDE-2-ImP5+ (AEOL 10150) on outcome from spinal cord compression (SCC) in the mouse. C57BL/6J mice were subjected to 60 min thoracic SCC after discontinuation of halothane anesthesia. In Experiment 1, mice were given intravenous Mn(III)TDE-2-ImP5+ (0.5 mg/kg bolus followed by 1 mg kg(-1) h(-1) for 24 h), methylprednisolone (30 mg/kg bolus followed by 5.4 mg kg(-1) h(-1) for 24 h), or vehicle (n = 25 per group). In Experiment 2, mice were given intrathecal Mn(III)TDE-2-ImP5+ (2.5 or 5.0 microg/kg) or vehicle (n = 18 per group). In both experiments, treatment began 5 min post-SCC onset. Rotarod performance was measured on post-SCC days 3, 7, 14, and 21. On post-SCC day 21, the spinal cord was histologically examined and a total damage score was calculated. Neither intravenous Mn(III)TDE-2-ImP5+ nor methylprednisolone altered rotarod performance (accelerated rate P = 0.11, fixed rate P = 0.11) or mean +/- S.D. total damage score (Mn(III)TDE-2-ImP5+ = 21 +/- 9, methylprednisolone = 24 +/- 8, vehicle = 22 +/- 10; P = 0.47; shams = 0). Intrathecal Mn(III)TDE-2-ImP5+ (both 2.5 and 5.0 microg) given at SCC-onset improved rotarod performance (P = 0.05) and total damage score (2.5 microg = 19 +/- 10, P = 0.04; 5.0 microg =19 +/- 8, P = 0.03) versus vehicle (26 +/- 10). These studies demonstrate sustained benefit from manganese(III) porphyrin catalytic antioxidant therapy after SCC. However, efficacy was dependent upon route of administration suggesting that bioavailability is critical in defining efficacy.

Duke Scholars

Published In

Neurosci Lett

DOI

ISSN

0304-3940

Publication Date

August 12, 2004

Volume

366

Issue

2

Start / End Page

220 / 225

Location

Ireland

Related Subject Headings

  • Spinal Cord Compression
  • Mice, Inbred C57BL
  • Mice
  • Metalloporphyrins
  • Injections, Spinal
  • Injections, Intravenous
  • Catalysis
  • Antioxidants
  • Animals
  • 5202 Biological psychology
 

Citation

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Sheng, H., Spasojevic, I., Warner, D. S., & Batinic-Haberle, I. (2004). Mouse spinal cord compression injury is ameliorated by intrathecal cationic manganese(III) porphyrin catalytic antioxidant therapy. Neurosci Lett, 366(2), 220–225. https://doi.org/10.1016/j.neulet.2004.05.050
Sheng, Huaxin, Ivan Spasojevic, David S. Warner, and Ines Batinic-Haberle. “Mouse spinal cord compression injury is ameliorated by intrathecal cationic manganese(III) porphyrin catalytic antioxidant therapy.Neurosci Lett 366, no. 2 (August 12, 2004): 220–25. https://doi.org/10.1016/j.neulet.2004.05.050.
Sheng H, Spasojevic I, Warner DS, Batinic-Haberle I. Mouse spinal cord compression injury is ameliorated by intrathecal cationic manganese(III) porphyrin catalytic antioxidant therapy. Neurosci Lett. 2004 Aug 12;366(2):220–5.
Sheng, Huaxin, et al. “Mouse spinal cord compression injury is ameliorated by intrathecal cationic manganese(III) porphyrin catalytic antioxidant therapy.Neurosci Lett, vol. 366, no. 2, Aug. 2004, pp. 220–25. Pubmed, doi:10.1016/j.neulet.2004.05.050.
Sheng H, Spasojevic I, Warner DS, Batinic-Haberle I. Mouse spinal cord compression injury is ameliorated by intrathecal cationic manganese(III) porphyrin catalytic antioxidant therapy. Neurosci Lett. 2004 Aug 12;366(2):220–225.
Journal cover image

Published In

Neurosci Lett

DOI

ISSN

0304-3940

Publication Date

August 12, 2004

Volume

366

Issue

2

Start / End Page

220 / 225

Location

Ireland

Related Subject Headings

  • Spinal Cord Compression
  • Mice, Inbred C57BL
  • Mice
  • Metalloporphyrins
  • Injections, Spinal
  • Injections, Intravenous
  • Catalysis
  • Antioxidants
  • Animals
  • 5202 Biological psychology