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The role of calcium in the regulation of prostacyclin synthesis by porcine aortic endothelial cells.

Publication ,  Journal Article
Whorton, AR; Willis, CE; Kent, RS; Young, SL
Published in: Lipids
January 1984

Both bradykinin (EC50 = 8 ng/ml) and the ionophore A23187 (EC50 = 3 X 10(-7) M) potently stimulated arachidonate release and prostaglandin synthesis in porcine aortic endothelial cells. The response to each was completely dependent on extracellular Ca2+ (EC50 = 3 X 10(-7) M); no role for intracellular Ca2+ was noted. The rapid Ca2+ influx prompted by either activator was consistent with the time course for arachidonate release. Whereas the arachidonate released in response to bradykinin was transient, that released in response to A23187 was more prolonged, and paralleled a continued influx of Ca2+. Ca2+ entry elicited by bradykinin was mediated by channels which could not be blocked by verapamil. When Mn2+ was substituted for Ca2+, no stimulation of prostacyclin synthesis was seen in response to A23187; however, the bradykinin response was unaffected. The mechanism of these effects was studied using doses of bradykinin or A23187 which resulted in increases in Ca2+ influx and prostacyclin synthesis of similar magnitude for each agonist. Under these conditions, trifluoperazine blocked elevated prostacyclin synthesis (ID50 = 5-6 X 10(-6) M for each agonist). Trifluoperazine sulfoxide, however, was much less active. Pimozide inhibited bradykinin-stimulated prostacyclin synthesis at low doses (ID50 = 3 X 10(-6) M). Trifluoperazine was much less effective against high doses of A23187 (4 X 10(-6) M). These data suggest that arachidonate release and prostacyclin synthesis are dependent on influx of extracellular calcium and subsequent activation of a Ca2+-dependent phospholipase by a calmodulin-mediated mechanism.

Duke Scholars

Published In

Lipids

DOI

ISSN

0024-4201

Publication Date

January 1984

Volume

19

Issue

1

Start / End Page

17 / 24

Location

United States

Related Subject Headings

  • Verapamil
  • Swine
  • Nutrition & Dietetics
  • Manganese
  • Epoprostenol
  • Endothelium
  • Calcium
  • Calcimycin
  • Bradykinin
  • Arachidonic Acids
 

Citation

APA
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ICMJE
MLA
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Whorton, A. R., Willis, C. E., Kent, R. S., & Young, S. L. (1984). The role of calcium in the regulation of prostacyclin synthesis by porcine aortic endothelial cells. Lipids, 19(1), 17–24. https://doi.org/10.1007/BF02534603
Whorton, A. R., C. E. Willis, R. S. Kent, and S. L. Young. “The role of calcium in the regulation of prostacyclin synthesis by porcine aortic endothelial cells.Lipids 19, no. 1 (January 1984): 17–24. https://doi.org/10.1007/BF02534603.
Whorton AR, Willis CE, Kent RS, Young SL. The role of calcium in the regulation of prostacyclin synthesis by porcine aortic endothelial cells. Lipids. 1984 Jan;19(1):17–24.
Whorton, A. R., et al. “The role of calcium in the regulation of prostacyclin synthesis by porcine aortic endothelial cells.Lipids, vol. 19, no. 1, Jan. 1984, pp. 17–24. Pubmed, doi:10.1007/BF02534603.
Whorton AR, Willis CE, Kent RS, Young SL. The role of calcium in the regulation of prostacyclin synthesis by porcine aortic endothelial cells. Lipids. 1984 Jan;19(1):17–24.
Journal cover image

Published In

Lipids

DOI

ISSN

0024-4201

Publication Date

January 1984

Volume

19

Issue

1

Start / End Page

17 / 24

Location

United States

Related Subject Headings

  • Verapamil
  • Swine
  • Nutrition & Dietetics
  • Manganese
  • Epoprostenol
  • Endothelium
  • Calcium
  • Calcimycin
  • Bradykinin
  • Arachidonic Acids