Skip to main content

Production and characterization of two human glioma xenograft-localizing monoclonal antibodies.

Publication ,  Journal Article
Wikstrand, CJ; McLendon, RE; Bullard, DE; Fredman, P; Svennerholm, L; Bigner, DD
Published in: Cancer Res
November 1986

Multiple fusions following immunization of athymic mice with the extensively characterized human glioma cell line D-54 MG resulted in the selection of several antibodies (Mabs) highly reactive with tumors of neuroectodermal origin and unreactive with normal nervous system tissue. Two Mabs, C12 and D12, which localized specifically to tumors in athymic mouse-human glioma xenograft paired label localization assays, are IgG3 antibodies; both bind readily to staphylococcal protein A in column purification and radioimmunoprecipitation procedures. Both iodinate via the chloramine-T method yielding 125I-immunoreactive product by direct cell surface radioimmunoassay and absorption assay. By indirect cell surface radioimmunoassay, a cultured cell line panel consisting of 17 gliomas, 3 medulloblastomas, 2 neuroblastomas, 2 melanomas, and 2 fetal and 2 adult brain-derived cell lines was examined; the two Mabs were highly similar but distinct in their reactivity profiles. Each was positive with greater than 47% of the gliomas tested (C12, 9 of 17; D12, 8 of 17); and with 1 of 3 medulloblastomas, 1 of 2 melanomas, and cell lines derived from 12- and 16-week-gestation human fetal brain. No reactivity was observed with neuroblastoma or adult brain-derived cell lines or with neutral glycolipids and gangliosides extracted from D-54 MG xenografts or human glioma cell lines. Notable extraneuroectodermal reactivity included that of Mab D12 for splenic trabeculae and the spermatids and Sertoli cells in the testes. Following immunoprecipitation of [3H]leucine labeled cell membrane preparations, Mabs C12 and D12 have consistently yielded unique bands in the Mr 180,000 and Mr 88,000 regions respectively. When used in paired label localization experiments in s.c. D-54 MG xenograft-bearing athymic mice, Mabs C12 and D12 demonstrate similar localization patterns, attaining peak localization indices at day 3 (D12) or 4 (C12); the maximum percentage of injected Mab bound to tumor ranged from 5% (D12) to 8% (C12). The peak tumor/normal brain localization ratios (167-181) attained by these Mabs at days 1-2 followed by their rapid clearance suggest that these Mabs are potentially useful imaging and therapeutic agents for further investigation.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

November 1986

Volume

46

Issue

11

Start / End Page

5933 / 5940

Location

United States

Related Subject Headings

  • Tissue Distribution
  • Oncology & Carcinogenesis
  • Neoplasm Transplantation
  • Molecular Weight
  • Mice, Nude
  • Mice
  • Medulloblastoma
  • Humans
  • Glioma
  • Cell Line
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wikstrand, C. J., McLendon, R. E., Bullard, D. E., Fredman, P., Svennerholm, L., & Bigner, D. D. (1986). Production and characterization of two human glioma xenograft-localizing monoclonal antibodies. Cancer Res, 46(11), 5933–5940.
Wikstrand, C. J., R. E. McLendon, D. E. Bullard, P. Fredman, L. Svennerholm, and D. D. Bigner. “Production and characterization of two human glioma xenograft-localizing monoclonal antibodies.Cancer Res 46, no. 11 (November 1986): 5933–40.
Wikstrand CJ, McLendon RE, Bullard DE, Fredman P, Svennerholm L, Bigner DD. Production and characterization of two human glioma xenograft-localizing monoclonal antibodies. Cancer Res. 1986 Nov;46(11):5933–40.
Wikstrand, C. J., et al. “Production and characterization of two human glioma xenograft-localizing monoclonal antibodies.Cancer Res, vol. 46, no. 11, Nov. 1986, pp. 5933–40.
Wikstrand CJ, McLendon RE, Bullard DE, Fredman P, Svennerholm L, Bigner DD. Production and characterization of two human glioma xenograft-localizing monoclonal antibodies. Cancer Res. 1986 Nov;46(11):5933–5940.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

November 1986

Volume

46

Issue

11

Start / End Page

5933 / 5940

Location

United States

Related Subject Headings

  • Tissue Distribution
  • Oncology & Carcinogenesis
  • Neoplasm Transplantation
  • Molecular Weight
  • Mice, Nude
  • Mice
  • Medulloblastoma
  • Humans
  • Glioma
  • Cell Line