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Systemic soluble Tie2 expression inhibits and regresses corneal neovascularization.

Publication ,  Journal Article
Singh, N; Macnamara, E; Rashid, S; Ambati, J; Kontos, CD; Higgins, E; Ambati, BK
Published in: Biochem Biophys Res Commun
June 24, 2005

This study was designed to determine if soluble Tie2 (sTie2) expression inhibits and regresses corneal neovascularization, and if VEGF contributes to its effect. The corneas of BALB/c mice were scraped and the mice were injected with either an adenovirus expressing soluble Tie2 (Ad.sTie2) or an empty adenoviral vector. When injected at the inhibition timepoint (one day prior to corneal injury), the mean percentage of neovascularized corneal area two weeks later in Ad.sTie2-treated mice vs. controls was 56.37+/-9.15% vs. 85.79+/-3.55% (p=0.04). At the regression timepoint (4 weeks after corneal scrape), the mean area of corneal neovascularization in Ad.sTie2-treated mice was 42.89+/-4.74% vs. 75.01+/-3.22% in the control group (p=0.007). VEGF expression was significantly higher in Ad.sTie2-treated mice at the inhibition timepoint and there was no significant difference at the regression timepoint. These findings suggest that sTie2 inhibits and regresses corneal neovascularization in a VEGF-independent manner.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

June 24, 2005

Volume

332

Issue

1

Start / End Page

194 / 199

Location

United States

Related Subject Headings

  • Vascular Endothelial Growth Factor A
  • Transfection
  • Solubility
  • Receptor, TIE-2
  • Mice, Inbred BALB C
  • Mice
  • Men
  • In Vitro Techniques
  • Corneal Neovascularization
  • Biochemistry & Molecular Biology
 

Citation

APA
Chicago
ICMJE
MLA
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Singh, N., Macnamara, E., Rashid, S., Ambati, J., Kontos, C. D., Higgins, E., & Ambati, B. K. (2005). Systemic soluble Tie2 expression inhibits and regresses corneal neovascularization. Biochem Biophys Res Commun, 332(1), 194–199. https://doi.org/10.1016/j.bbrc.2005.04.108
Singh, Nirbhai, Elizabeth Macnamara, Saadia Rashid, Jayakrishna Ambati, Christopher D. Kontos, Eric Higgins, and Balamurali K. Ambati. “Systemic soluble Tie2 expression inhibits and regresses corneal neovascularization.Biochem Biophys Res Commun 332, no. 1 (June 24, 2005): 194–99. https://doi.org/10.1016/j.bbrc.2005.04.108.
Singh N, Macnamara E, Rashid S, Ambati J, Kontos CD, Higgins E, et al. Systemic soluble Tie2 expression inhibits and regresses corneal neovascularization. Biochem Biophys Res Commun. 2005 Jun 24;332(1):194–9.
Singh, Nirbhai, et al. “Systemic soluble Tie2 expression inhibits and regresses corneal neovascularization.Biochem Biophys Res Commun, vol. 332, no. 1, June 2005, pp. 194–99. Pubmed, doi:10.1016/j.bbrc.2005.04.108.
Singh N, Macnamara E, Rashid S, Ambati J, Kontos CD, Higgins E, Ambati BK. Systemic soluble Tie2 expression inhibits and regresses corneal neovascularization. Biochem Biophys Res Commun. 2005 Jun 24;332(1):194–199.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

June 24, 2005

Volume

332

Issue

1

Start / End Page

194 / 199

Location

United States

Related Subject Headings

  • Vascular Endothelial Growth Factor A
  • Transfection
  • Solubility
  • Receptor, TIE-2
  • Mice, Inbred BALB C
  • Mice
  • Men
  • In Vitro Techniques
  • Corneal Neovascularization
  • Biochemistry & Molecular Biology