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Aquaporins in Saccharomyces. Genetic and functional distinctions between laboratory and wild-type strains.

Publication ,  Journal Article
Bonhivers, M; Carbrey, JM; Gould, SJ; Agre, P
Published in: J Biol Chem
October 16, 1998

Aquaporin water channel proteins mediate the transport of water across cell membranes in numerous species. The Saccharomyces genome data base contains an open reading frame (here designated AQY1) that encodes a protein with strong homology to aquaporins. AQY1 from laboratory and wild-type strains of Saccharomyces were expressed in Xenopus oocytes to determine the coefficients of osmotic water permeability (Pf). Oocytes injected with wild-type AQY1 cRNAs exhibit high Pf values, whereas oocytes injected with AQY1 cRNAs from laboratory strains exhibit low Pf values and have reduced levels of Aqy1p due to two amino acid substitutions. When the AQY1 gene was deleted from a wild-type yeast and cells were cultured in vitro with cycled hypo-osmolar or hyper-osmolar stresses, the AQY1 null yeast showed significantly improved viability when compared with the parental wild-type strain. We conclude that Saccharomyces cerevisiae contains at least one aquaporin gene, but it is not functional in laboratory strains due to apparent negative selection pressures resulting from in vitro methods.

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Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

October 16, 1998

Volume

273

Issue

42

Start / End Page

27565 / 27572

Location

United States

Related Subject Headings

  • Xenopus
  • Water
  • Sequence Homology, Amino Acid
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Recombinant Proteins
  • Phylogeny
  • Osmotic Pressure
  • Oocytes
  • Mutagenesis
 

Citation

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Bonhivers, M., Carbrey, J. M., Gould, S. J., & Agre, P. (1998). Aquaporins in Saccharomyces. Genetic and functional distinctions between laboratory and wild-type strains. J Biol Chem, 273(42), 27565–27572. https://doi.org/10.1074/jbc.273.42.27565
Bonhivers, M., J. M. Carbrey, S. J. Gould, and P. Agre. “Aquaporins in Saccharomyces. Genetic and functional distinctions between laboratory and wild-type strains.J Biol Chem 273, no. 42 (October 16, 1998): 27565–72. https://doi.org/10.1074/jbc.273.42.27565.
Bonhivers M, Carbrey JM, Gould SJ, Agre P. Aquaporins in Saccharomyces. Genetic and functional distinctions between laboratory and wild-type strains. J Biol Chem. 1998 Oct 16;273(42):27565–72.
Bonhivers, M., et al. “Aquaporins in Saccharomyces. Genetic and functional distinctions between laboratory and wild-type strains.J Biol Chem, vol. 273, no. 42, Oct. 1998, pp. 27565–72. Pubmed, doi:10.1074/jbc.273.42.27565.
Bonhivers M, Carbrey JM, Gould SJ, Agre P. Aquaporins in Saccharomyces. Genetic and functional distinctions between laboratory and wild-type strains. J Biol Chem. 1998 Oct 16;273(42):27565–27572.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

October 16, 1998

Volume

273

Issue

42

Start / End Page

27565 / 27572

Location

United States

Related Subject Headings

  • Xenopus
  • Water
  • Sequence Homology, Amino Acid
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Recombinant Proteins
  • Phylogeny
  • Osmotic Pressure
  • Oocytes
  • Mutagenesis