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Profiling of CD4+, CD8+, and CD4+CD25+CD45RO+FoxP3+ T cells in patients with malignant glioma reveals differential expression of the immunologic transcriptome compared with T cells from healthy volunteers.

Publication ,  Journal Article
Learn, CA; Fecci, PE; Schmittling, RJ; Xie, W; Karikari, I; Mitchell, DA; Archer, GE; Wei, Z; Dressman, H; Sampson, JH
Published in: Clin Cancer Res
December 15, 2006

PURPOSE: Analyses of T-cell mRNA expression profiles in glioblastoma multiforme has not been previously reported but may help to define and characterize the immunosuppressed phenotype in patients with this type of cancer. EXPERIMENTAL DESIGN: We did microarray studies that have shown significant and fundamental differences in the expression profiles of CD4(+) and CD8(+) T cells and immunosuppressive CD4(+)CD25(+)CD45RO(+)FoxP3(+) regulatory T cells (T(reg)) from normal healthy volunteers compared with patients with newly diagnosed glioblastoma multiforme. For these investigations, we isolated total RNA from enriched CD4(+) and CD8(+) T cell or T(reg) cell populations from age-matched individuals and did microarray analyses. RESULTS: ANOVA and principal components analysis show that the various T cell compartments exhibit consistently similar mRNA expression profiles among individuals within either healthy or brain tumor groups but reflect significant differences between these groups. Compared with healthy volunteers, CD4(+) and CD8(+) T cells from patients with glioblastoma multiforme display coordinate down-regulation of genes involved in T cell receptor ligation, activation, and intracellular signaling. In contrast, T(regs) from patients with glioblastoma multiforme exhibit increased levels of transcripts involved in inhibiting host immunity. CONCLUSION: Our findings support the notion that key differences between expression profiles in T-cell populations from patients with glioblastoma multiforme results from differential expression of the immunologic transcriptome, such that a limited number of genes are principally important in producing the dysregulated T-cell phenotype.

Duke Scholars

Published In

Clin Cancer Res

DOI

ISSN

1078-0432

Publication Date

December 15, 2006

Volume

12

Issue

24

Start / End Page

7306 / 7315

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Oncology & Carcinogenesis
  • Middle Aged
  • Male
  • Leukocyte Common Antigens
  • Interleukin-2 Receptor alpha Subunit
  • Immunophenotyping
  • Humans
  • Glioma
  • Glioblastoma
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Learn, C. A., Fecci, P. E., Schmittling, R. J., Xie, W., Karikari, I., Mitchell, D. A., … Sampson, J. H. (2006). Profiling of CD4+, CD8+, and CD4+CD25+CD45RO+FoxP3+ T cells in patients with malignant glioma reveals differential expression of the immunologic transcriptome compared with T cells from healthy volunteers. Clin Cancer Res, 12(24), 7306–7315. https://doi.org/10.1158/1078-0432.CCR-06-1727
Learn, Chris A., Peter E. Fecci, Robert J. Schmittling, Weihua Xie, Isaac Karikari, Duane A. Mitchell, Gary E. Archer, ZhengZheng Wei, Holly Dressman, and John H. Sampson. “Profiling of CD4+, CD8+, and CD4+CD25+CD45RO+FoxP3+ T cells in patients with malignant glioma reveals differential expression of the immunologic transcriptome compared with T cells from healthy volunteers.Clin Cancer Res 12, no. 24 (December 15, 2006): 7306–15. https://doi.org/10.1158/1078-0432.CCR-06-1727.
Learn CA, Fecci PE, Schmittling RJ, Xie W, Karikari I, Mitchell DA, Archer GE, Wei Z, Dressman H, Sampson JH. Profiling of CD4+, CD8+, and CD4+CD25+CD45RO+FoxP3+ T cells in patients with malignant glioma reveals differential expression of the immunologic transcriptome compared with T cells from healthy volunteers. Clin Cancer Res. 2006 Dec 15;12(24):7306–7315.

Published In

Clin Cancer Res

DOI

ISSN

1078-0432

Publication Date

December 15, 2006

Volume

12

Issue

24

Start / End Page

7306 / 7315

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Oncology & Carcinogenesis
  • Middle Aged
  • Male
  • Leukocyte Common Antigens
  • Interleukin-2 Receptor alpha Subunit
  • Immunophenotyping
  • Humans
  • Glioma
  • Glioblastoma