Skip to main content
Journal cover image

Age-related studies of SIg, Leu-4 and concanavalin A receptor densities and capping in human lymphocytes.

Publication ,  Journal Article
Cohen, HJ; Boland, KM; Rao, KM
Published in: Mech Ageing Dev
June 28, 1991

We compared the cell surface antigen density and capping of three antigens in lymphocytes obtained from healthy, young (mean age 27 years) and elderly (mean age 76), population. There were no differences in the expression of surface immunoglobulin (SIg), concanavalin A (con A) receptors and Leu-4 antigen between the two groups. Kinetic analysis of these molecules revealed a slight decrease in capping in the older population, but the differences were not statistically significant. In order to test the possibility that subjecting the cells to metabolic stress might bring out the differences, we performed a kinetic analysis of SIg and con A capping in the presence of various concentrations of the metabolic inhibitor sodium azide. Although the capping in cells from elderly subjects was slightly more sensitive to azide, no statistical difference was found. Analysis of con A capping by a flow cytometric method yielded similar results, confirming the data obtained by visual capping experiments. We conclude that although a trend toward decreased capping was observed, there is little alteration in the surface molecule capping phenomenon in the age-groups studied.

Duke Scholars

Published In

Mech Ageing Dev

DOI

ISSN

0047-6374

Publication Date

June 28, 1991

Volume

59

Issue

3

Start / End Page

253 / 262

Location

Ireland

Related Subject Headings

  • Sodium Azide
  • Receptors, Immunologic
  • Receptors, Antigen, B-Cell
  • Lymphocytes
  • Immunologic Capping
  • Humans
  • Gerontology
  • Flow Cytometry
  • Concanavalin A
  • Azides
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Cohen, H. J., Boland, K. M., & Rao, K. M. (1991). Age-related studies of SIg, Leu-4 and concanavalin A receptor densities and capping in human lymphocytes. Mech Ageing Dev, 59(3), 253–262. https://doi.org/10.1016/0047-6374(91)90136-n
Cohen, H. J., K. M. Boland, and K. M. Rao. “Age-related studies of SIg, Leu-4 and concanavalin A receptor densities and capping in human lymphocytes.Mech Ageing Dev 59, no. 3 (June 28, 1991): 253–62. https://doi.org/10.1016/0047-6374(91)90136-n.
Cohen HJ, Boland KM, Rao KM. Age-related studies of SIg, Leu-4 and concanavalin A receptor densities and capping in human lymphocytes. Mech Ageing Dev. 1991 Jun 28;59(3):253–62.
Cohen, H. J., et al. “Age-related studies of SIg, Leu-4 and concanavalin A receptor densities and capping in human lymphocytes.Mech Ageing Dev, vol. 59, no. 3, June 1991, pp. 253–62. Pubmed, doi:10.1016/0047-6374(91)90136-n.
Cohen HJ, Boland KM, Rao KM. Age-related studies of SIg, Leu-4 and concanavalin A receptor densities and capping in human lymphocytes. Mech Ageing Dev. 1991 Jun 28;59(3):253–262.
Journal cover image

Published In

Mech Ageing Dev

DOI

ISSN

0047-6374

Publication Date

June 28, 1991

Volume

59

Issue

3

Start / End Page

253 / 262

Location

Ireland

Related Subject Headings

  • Sodium Azide
  • Receptors, Immunologic
  • Receptors, Antigen, B-Cell
  • Lymphocytes
  • Immunologic Capping
  • Humans
  • Gerontology
  • Flow Cytometry
  • Concanavalin A
  • Azides