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Nitrosylation of blood hemoglobin and renal nonheme proteins in autoimmune MRL-lpr/lpr mice.

Publication ,  Journal Article
Weinberg, JB; Gilkeson, GS; Mason, RP; Chamulitrat, W
Published in: Free Radic Biol Med
January 1, 1998

MRL-lpr/lpr mice spontaneously develop manifestations of autoimmunity including arthritis, vasculitis, and glomerulonephritis. The paramagnetic molecule nitric oxide has been implicated as an effector molecule in initiation and propagation of these inflammatory conditions. In this study, we utilized electron paramagnetic resonance spectroscopy to directly detect nitrosylated protein complexes as products of nitric oxide in whole blood and in kidneys of MRL-lpr/lpr mice. Electron paramagnetic resonance spectra of blood samples from MRL-lpr/lpr mice showed nitrosyl hemoglobin species. Amounts of blood nitrosyl hemoglobin in MRL-lpr/lpr mice were significantly increased as compared to age-matched control mice. Electron paramagnetic resonance spectra of MRL-lpr/lpr kidney tissue exhibited a signal characteristic of a dinitrosyl-iron-dithiolate complex at g approximately 2.04. Formation of nitrosylated nonheme protein in diseased kidneys is associated with development of glomerulonephritis in the autoimmune mice. The presence of nitrosylated nonheme protein indicates the formation of nitric oxide within the kidneys of the diseased mice signifying in situ renal nitric oxide formation.

Duke Scholars

Published In

Free Radic Biol Med

DOI

ISSN

0891-5849

Publication Date

January 1, 1998

Volume

24

Issue

1

Start / End Page

191 / 196

Location

United States

Related Subject Headings

  • Nonheme Iron Proteins
  • Nitric Oxide
  • Mice, Inbred Strains
  • Mice
  • Kidney
  • Hemoglobins
  • Disease Models, Animal
  • Biochemistry & Molecular Biology
  • Autoimmune Diseases
  • Animals
 

Citation

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Weinberg, J. B., Gilkeson, G. S., Mason, R. P., & Chamulitrat, W. (1998). Nitrosylation of blood hemoglobin and renal nonheme proteins in autoimmune MRL-lpr/lpr mice. Free Radic Biol Med, 24(1), 191–196. https://doi.org/10.1016/s0891-5849(97)00217-7
Weinberg, J. B., G. S. Gilkeson, R. P. Mason, and W. Chamulitrat. “Nitrosylation of blood hemoglobin and renal nonheme proteins in autoimmune MRL-lpr/lpr mice.Free Radic Biol Med 24, no. 1 (January 1, 1998): 191–96. https://doi.org/10.1016/s0891-5849(97)00217-7.
Weinberg JB, Gilkeson GS, Mason RP, Chamulitrat W. Nitrosylation of blood hemoglobin and renal nonheme proteins in autoimmune MRL-lpr/lpr mice. Free Radic Biol Med. 1998 Jan 1;24(1):191–6.
Weinberg, J. B., et al. “Nitrosylation of blood hemoglobin and renal nonheme proteins in autoimmune MRL-lpr/lpr mice.Free Radic Biol Med, vol. 24, no. 1, Jan. 1998, pp. 191–96. Pubmed, doi:10.1016/s0891-5849(97)00217-7.
Weinberg JB, Gilkeson GS, Mason RP, Chamulitrat W. Nitrosylation of blood hemoglobin and renal nonheme proteins in autoimmune MRL-lpr/lpr mice. Free Radic Biol Med. 1998 Jan 1;24(1):191–196.
Journal cover image

Published In

Free Radic Biol Med

DOI

ISSN

0891-5849

Publication Date

January 1, 1998

Volume

24

Issue

1

Start / End Page

191 / 196

Location

United States

Related Subject Headings

  • Nonheme Iron Proteins
  • Nitric Oxide
  • Mice, Inbred Strains
  • Mice
  • Kidney
  • Hemoglobins
  • Disease Models, Animal
  • Biochemistry & Molecular Biology
  • Autoimmune Diseases
  • Animals