Skip to main content

Schedule and concentration-dependent induction of apoptosis in leukemia cells by nitric oxide.

Publication ,  Journal Article
Shami, PJ; Sauls, DL; Weinberg, JB
Published in: Leukemia
September 1998

Nitric oxide (NO) has potent antiproliferative properties. In previous work we have shown that NO inhibits growth, induces differentiation and modulates gene expression in acute nonlymphocytic leukemia (ANLL) cells. The goal of this work was to determine whether the rate of NO delivery affected its growth inhibition of ANLL cells. We also wanted to determine whether the NO inhibition of ANLL cell growth is associated with the induction of apoptosis. We treated HL-60 and U937 cells with three compounds that generate the same amount of NO but at different rates. MAMA-NO, PAPA-NO and DETA-NO have half-lives of NO delivery of 2 and 30 min, and 20 h, respectively. The compound with the longest t(1/2) of NO delivery (DETA-NO) was the most potent inhibitor of leukemia cell and colony growth. Furthermore, the NO-induced growth inhibition was associated with apoptosis in a rate and concentration-dependent fashion.

Duke Scholars

Published In

Leukemia

DOI

ISSN

0887-6924

Publication Date

September 1998

Volume

12

Issue

9

Start / End Page

1461 / 1466

Location

England

Related Subject Headings

  • Tumor Stem Cell Assay
  • Tumor Cells, Cultured
  • Nitric Oxide
  • Leukemia, Myeloid, Acute
  • Immunology
  • Humans
  • HL-60 Cells
  • Dose-Response Relationship, Drug
  • Cell Division
  • Apoptosis
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Shami, P. J., Sauls, D. L., & Weinberg, J. B. (1998). Schedule and concentration-dependent induction of apoptosis in leukemia cells by nitric oxide. Leukemia, 12(9), 1461–1466. https://doi.org/10.1038/sj.leu.2401131
Shami, P. J., D. L. Sauls, and J. B. Weinberg. “Schedule and concentration-dependent induction of apoptosis in leukemia cells by nitric oxide.Leukemia 12, no. 9 (September 1998): 1461–66. https://doi.org/10.1038/sj.leu.2401131.
Shami PJ, Sauls DL, Weinberg JB. Schedule and concentration-dependent induction of apoptosis in leukemia cells by nitric oxide. Leukemia. 1998 Sep;12(9):1461–6.
Shami, P. J., et al. “Schedule and concentration-dependent induction of apoptosis in leukemia cells by nitric oxide.Leukemia, vol. 12, no. 9, Sept. 1998, pp. 1461–66. Pubmed, doi:10.1038/sj.leu.2401131.
Shami PJ, Sauls DL, Weinberg JB. Schedule and concentration-dependent induction of apoptosis in leukemia cells by nitric oxide. Leukemia. 1998 Sep;12(9):1461–1466.

Published In

Leukemia

DOI

ISSN

0887-6924

Publication Date

September 1998

Volume

12

Issue

9

Start / End Page

1461 / 1466

Location

England

Related Subject Headings

  • Tumor Stem Cell Assay
  • Tumor Cells, Cultured
  • Nitric Oxide
  • Leukemia, Myeloid, Acute
  • Immunology
  • Humans
  • HL-60 Cells
  • Dose-Response Relationship, Drug
  • Cell Division
  • Apoptosis