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Syne-1 and Syne-2 play crucial roles in myonuclear anchorage and motor neuron innervation.

Publication ,  Journal Article
Zhang, X; Xu, R; Zhu, B; Yang, X; Ding, X; Duan, S; Xu, T; Zhuang, Y; Han, M
Published in: Development
March 2007

Proper nuclear positioning is important to cell function in many biological processes during animal development. In certain cells, the KASH-domain-containing proteins have been shown to be associated with the nuclear envelope, and to be involved in both nuclear anchorage and migration. We investigated the mechanism and function of nuclear anchorage in skeletal muscle cells by generating mice with single and double-disruption of the KASH-domain-containing genes Syne1 (also known as Syne-1) and Syne2 (also known as Syne-2). We showed that the deletion of the KASH domain of Syne-1 abolished the formation of clusters of synaptic nuclei and disrupted the organization of non-synaptic nuclei in skeletal muscle. Further analysis indicated that the loss of synaptic nuclei in Syne-1 KASH-knockout mice significantly affected the innervation sites and caused longer motor nerve branches. Although disruption of neither Syne-1 nor Syne-2 affected viability or fertility, Syne-1; Syne-2 double-knockout mice died of respiratory failure within 20 minutes of birth. These results suggest that the KASH-domain-containing proteins Syne-1 and Syne-2 play crucial roles in anchoring both synaptic and non-synaptic myonuclei that are important for proper motor neuron innervation and respiration.

Duke Scholars

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Published In

Development

DOI

ISSN

0950-1991

Publication Date

March 2007

Volume

134

Issue

5

Start / End Page

901 / 908

Location

England

Related Subject Headings

  • Synapses
  • Protein Structure, Tertiary
  • Nuclear Proteins
  • Neuromuscular Junction
  • Nerve Tissue Proteins
  • Muscle, Skeletal
  • Motor Neurons
  • Mice, Knockout
  • Mice
  • Cytoskeletal Proteins
 

Citation

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Zhang, X., Xu, R., Zhu, B., Yang, X., Ding, X., Duan, S., … Han, M. (2007). Syne-1 and Syne-2 play crucial roles in myonuclear anchorage and motor neuron innervation. Development, 134(5), 901–908. https://doi.org/10.1242/dev.02783
Zhang, Xiaochang, Rener Xu, Binggen Zhu, Xiujuan Yang, Xu Ding, Shumin Duan, Tian Xu, Yuan Zhuang, and Min Han. “Syne-1 and Syne-2 play crucial roles in myonuclear anchorage and motor neuron innervation.Development 134, no. 5 (March 2007): 901–8. https://doi.org/10.1242/dev.02783.
Zhang X, Xu R, Zhu B, Yang X, Ding X, Duan S, et al. Syne-1 and Syne-2 play crucial roles in myonuclear anchorage and motor neuron innervation. Development. 2007 Mar;134(5):901–8.
Zhang, Xiaochang, et al. “Syne-1 and Syne-2 play crucial roles in myonuclear anchorage and motor neuron innervation.Development, vol. 134, no. 5, Mar. 2007, pp. 901–08. Pubmed, doi:10.1242/dev.02783.
Zhang X, Xu R, Zhu B, Yang X, Ding X, Duan S, Xu T, Zhuang Y, Han M. Syne-1 and Syne-2 play crucial roles in myonuclear anchorage and motor neuron innervation. Development. 2007 Mar;134(5):901–908.
Journal cover image

Published In

Development

DOI

ISSN

0950-1991

Publication Date

March 2007

Volume

134

Issue

5

Start / End Page

901 / 908

Location

England

Related Subject Headings

  • Synapses
  • Protein Structure, Tertiary
  • Nuclear Proteins
  • Neuromuscular Junction
  • Nerve Tissue Proteins
  • Muscle, Skeletal
  • Motor Neurons
  • Mice, Knockout
  • Mice
  • Cytoskeletal Proteins