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Chromosomal translocation and segmental duplication in Cryptococcus neoformans.

Publication ,  Journal Article
Fraser, JA; Huang, JC; Pukkila-Worley, R; Alspaugh, JA; Mitchell, TG; Heitman, J
Published in: Eukaryot Cell
February 2005

Large chromosomal events such as translocations and segmental duplications enable rapid adaptation to new environments. Here we marshal genomic, genetic, meiotic mapping, and physical evidence to demonstrate that a chromosomal translocation and segmental duplication occurred during construction of a congenic strain pair in the fungal human pathogen Cryptococcus neoformans. Two chromosomes underwent telomere-telomere fusion, generating a dicentric chromosome that broke to produce a chromosomal translocation, forming two novel chromosomes sharing a large segmental duplication. The duplication spans 62,872 identical nucleotides and generated a second copy of 22 predicted genes, and we hypothesize that this event may have occurred during meiosis. Gene disruption studies of one embedded gene (SMG1) corroborate that this region is duplicated in an otherwise haploid genome. These findings resolve a genome project assembly anomaly and illustrate an example of rapid genome evolution in a fungal genome rich in repetitive elements.

Duke Scholars

Published In

Eukaryot Cell

DOI

ISSN

1535-9778

Publication Date

February 2005

Volume

4

Issue

2

Start / End Page

401 / 406

Location

United States

Related Subject Headings

  • Translocation, Genetic
  • Telomere
  • Molecular Sequence Data
  • Microbiology
  • Humans
  • Genome, Fungal
  • Gene Duplication
  • Fungal Proteins
  • Cryptococcus neoformans
  • Chromosomes, Fungal
 

Citation

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Fraser, J. A., Huang, J. C., Pukkila-Worley, R., Alspaugh, J. A., Mitchell, T. G., & Heitman, J. (2005). Chromosomal translocation and segmental duplication in Cryptococcus neoformans. Eukaryot Cell, 4(2), 401–406. https://doi.org/10.1128/EC.4.2.401-406.2005
Fraser, James A., Johnny C. Huang, Read Pukkila-Worley, J Andrew Alspaugh, Thomas G. Mitchell, and Joseph Heitman. “Chromosomal translocation and segmental duplication in Cryptococcus neoformans.Eukaryot Cell 4, no. 2 (February 2005): 401–6. https://doi.org/10.1128/EC.4.2.401-406.2005.
Fraser JA, Huang JC, Pukkila-Worley R, Alspaugh JA, Mitchell TG, Heitman J. Chromosomal translocation and segmental duplication in Cryptococcus neoformans. Eukaryot Cell. 2005 Feb;4(2):401–6.
Fraser, James A., et al. “Chromosomal translocation and segmental duplication in Cryptococcus neoformans.Eukaryot Cell, vol. 4, no. 2, Feb. 2005, pp. 401–06. Pubmed, doi:10.1128/EC.4.2.401-406.2005.
Fraser JA, Huang JC, Pukkila-Worley R, Alspaugh JA, Mitchell TG, Heitman J. Chromosomal translocation and segmental duplication in Cryptococcus neoformans. Eukaryot Cell. 2005 Feb;4(2):401–406.

Published In

Eukaryot Cell

DOI

ISSN

1535-9778

Publication Date

February 2005

Volume

4

Issue

2

Start / End Page

401 / 406

Location

United States

Related Subject Headings

  • Translocation, Genetic
  • Telomere
  • Molecular Sequence Data
  • Microbiology
  • Humans
  • Genome, Fungal
  • Gene Duplication
  • Fungal Proteins
  • Cryptococcus neoformans
  • Chromosomes, Fungal