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Human cystic fibrosis transmembrane conductance regulator directed to respiratory epithelial cells of transgenic mice.

Publication ,  Journal Article
Whitsett, JA; Dey, CR; Stripp, BR; Wikenheiser, KA; Clark, JC; Wert, SE; Gregory, RJ; Smith, AE; Cohn, JA; Wilson, JM
Published in: Nat Genet
September 1992
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Human cystic fibrosis transmembrane conductance regulator (CFTR) was expressed in transgenic mice under the control of transcriptional elements derived from the human surfactant protein C (SP-C) gene. The hCFTR mRNA was expressed in lungs and testes: in the lung, we found hCFTR mRNA in bronchiolar and alveolar epithelial cells, and CFTR protein in respiratory epithelial cells. While the level of expression of hCFTR mRNA varied, hCFTR mRNA and protein were detected in pulmonary epithelial cells of several lines. Lung weight, morphology, somatic growth and reproductive capacity were not altered by expression hCFTR in lung and testes of the transgenics. Our findings suggest that hCFTR can be safely transferred to lung epithelial cells for CF therapy.

Duke Scholars

Jonathan Allen Cohn
Author Jonathan Allen Cohn Medicine, Gastroenterology
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Published In

Nat Genet

DOI

10.1038/ng0992-13

ISSN

1061-4036

Publication Date

September 1992

Volume

2

Issue

1

Start / End Page

13 / 20

Location

United States

Related Subject Headings

  • Tissue Distribution
  • RNA, Messenger
  • Pulmonary Surfactants
  • Proteolipids
  • Mice, Transgenic
  • Mice
  • Membrane Proteins
  • Male
  • Lung
  • In Situ Hybridization
 

Citation

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Whitsett, J. A., Dey, C. R., Stripp, B. R., Wikenheiser, K. A., Clark, J. C., Wert, S. E., … Wilson, J. M. (1992). Human cystic fibrosis transmembrane conductance regulator directed to respiratory epithelial cells of transgenic mice. Nat Genet, 2(1), 13–20. https://doi.org/10.1038/ng0992-13
Whitsett, J. A., C. R. Dey, B. R. Stripp, K. A. Wikenheiser, J. C. Clark, S. E. Wert, R. J. Gregory, A. E. Smith, J. A. Cohn, and J. M. Wilson. “Human cystic fibrosis transmembrane conductance regulator directed to respiratory epithelial cells of transgenic mice.” Nat Genet 2, no. 1 (September 1992): 13–20. https://doi.org/10.1038/ng0992-13.
Whitsett JA, Dey CR, Stripp BR, Wikenheiser KA, Clark JC, Wert SE, et al. Human cystic fibrosis transmembrane conductance regulator directed to respiratory epithelial cells of transgenic mice. Nat Genet. 1992 Sep;2(1):13–20.
Whitsett, J. A., et al. “Human cystic fibrosis transmembrane conductance regulator directed to respiratory epithelial cells of transgenic mice.” Nat Genet, vol. 2, no. 1, Sept. 1992, pp. 13–20. Pubmed, doi:10.1038/ng0992-13.
Whitsett JA, Dey CR, Stripp BR, Wikenheiser KA, Clark JC, Wert SE, Gregory RJ, Smith AE, Cohn JA, Wilson JM. Human cystic fibrosis transmembrane conductance regulator directed to respiratory epithelial cells of transgenic mice. Nat Genet. 1992 Sep;2(1):13–20.

Published In

Nat Genet

DOI

10.1038/ng0992-13

ISSN

1061-4036

Publication Date

September 1992

Volume

2

Issue

1

Start / End Page

13 / 20

Location

United States

Related Subject Headings

  • Tissue Distribution
  • RNA, Messenger
  • Pulmonary Surfactants
  • Proteolipids
  • Mice, Transgenic
  • Mice
  • Membrane Proteins
  • Male
  • Lung
  • In Situ Hybridization