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Induction of cytotoxic T lymphocytes with dendritic cells transfected with human papillomavirus E6 and E7 RNA: implications for cervical cancer immunotherapy.

Publication ,  Journal Article
Thornburg, C; Boczkowski, D; Gilboa, E; Nair, SK
Published in: J Immunother
2000

Human papillomavirus (HPV) infection is associated with cervical cancer. The high-risk HPV E6 and E7 oncoproteins are constitutively expressed in most cervical carcinoma cells, and are, therefore, attractive antigens for cytotoxic T-lymphocyte (CTL)-mediated immunotherapy. The objective of this study was to evaluate the use of dendritic cells (DCs) transfected with RNA encoding the E6 and E7 protein for cervical cancer immunotherapy. The authors have shown that DCs transfected with RNA-encoding antigen stimulate potent antigen-specific CTL responses in vitro and in vivo. In this study, they tried to determine whether DCs transfected with E6 and E7 RNA stimulate primary, antigen-specific CTL responses in vitro. The results show that DCs pulsed with E6 or E7 RNA stimulate antigen-specific CTL responses that recognize and lyse DCs transfected with E6 and E7 RNA and human cervical carcinoma cells expressing the E6 and E7 products, and the lysis was comparable to that achieved with E6 and E7 peptide-pulsed DCs. Dendritic cells cotransfected with both E6 and E7 RNA stimulate CTLs that are more effective at lysing human cervical cancer cells. This study provides a rationale for the development of cervical carcinoma immunotherapy using DCs transfected with HPV E6 and E7 RNA.

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Published In

J Immunother

DOI

ISSN

1524-9557

Publication Date

2000

Volume

23

Issue

4

Start / End Page

412 / 418

Location

United States

Related Subject Headings

  • Uterine Cervical Neoplasms
  • Tumor Cells, Cultured
  • Transfection
  • T-Lymphocytes, Cytotoxic
  • Repressor Proteins
  • RNA, Viral
  • Peptides
  • Papillomavirus E7 Proteins
  • Oncogene Proteins, Viral
  • Immunotherapy, Adoptive
 

Citation

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Thornburg, C., Boczkowski, D., Gilboa, E., & Nair, S. K. (2000). Induction of cytotoxic T lymphocytes with dendritic cells transfected with human papillomavirus E6 and E7 RNA: implications for cervical cancer immunotherapy. J Immunother, 23(4), 412–418. https://doi.org/10.1097/00002371-200007000-00004
Thornburg, C., D. Boczkowski, E. Gilboa, and S. K. Nair. “Induction of cytotoxic T lymphocytes with dendritic cells transfected with human papillomavirus E6 and E7 RNA: implications for cervical cancer immunotherapy.J Immunother 23, no. 4 (2000): 412–18. https://doi.org/10.1097/00002371-200007000-00004.
Thornburg, C., et al. “Induction of cytotoxic T lymphocytes with dendritic cells transfected with human papillomavirus E6 and E7 RNA: implications for cervical cancer immunotherapy.J Immunother, vol. 23, no. 4, 2000, pp. 412–18. Pubmed, doi:10.1097/00002371-200007000-00004.

Published In

J Immunother

DOI

ISSN

1524-9557

Publication Date

2000

Volume

23

Issue

4

Start / End Page

412 / 418

Location

United States

Related Subject Headings

  • Uterine Cervical Neoplasms
  • Tumor Cells, Cultured
  • Transfection
  • T-Lymphocytes, Cytotoxic
  • Repressor Proteins
  • RNA, Viral
  • Peptides
  • Papillomavirus E7 Proteins
  • Oncogene Proteins, Viral
  • Immunotherapy, Adoptive