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Mouse TCRalphabeta+CD8alphaalpha intraepithelial lymphocytes express genes that down-regulate their antigen reactivity and suppress immune responses.

Publication ,  Journal Article
Denning, TL; Granger, SW; Mucida, D; Graddy, R; Leclercq, G; Zhang, W; Honey, K; Rasmussen, JP; Cheroutre, H; Rudensky, AY; Kronenberg, M
Published in: J Immunol
April 1, 2007

Mouse small intestine intraepithelial lymphocytes (IEL) that express alphabetaTCR and CD8alphaalpha homodimers are an enigmatic T cell subset, as their specificity and in vivo function remain to be defined. To gain insight into the nature of these cells, we performed global gene expression profiling using microarray analysis combined with real-time quantitative PCR and flow cytometry. Using these methods, TCRalphabeta(+)CD8alphaalpha IEL were compared with their TCRalphabeta(+)CD8beta(+) and TCRgammadelta(+) counterparts. Interestingly, TCRalphabeta(+)CD8alphaalpha IEL were found to preferentially express genes that would be expected to down-modulate their reactivity. They have a unique expression pattern of members of the Ly49 family of NK receptors and tend to express inhibitory receptors, along with some activating receptors. The signaling machinery of both TCRalphabeta(+)CD8alphaalpha and TCRgammadelta(+) IEL is constructed differently than other IEL and peripheral T cells, as evidenced by their low-level expression of the linker for activation of T cells and high expression of the non-T cell activation linker, which suppresses T cell activation. The TCRalphabeta(+)CD8alphaalpha IEL subset also has increased expression of genes that could be involved in immune regulation, including TGF-beta(3) and lymphocyte activation gene-3. Collectively, these data underscore the fact that, while TCRalphabeta(+)CD8alphaalpha IEL resemble TCRgammadelta(+) IEL, they are a unique population of cells with regulated Ag reactivity that could have regulatory function.

Duke Scholars

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

April 1, 2007

Volume

178

Issue

7

Start / End Page

4230 / 4239

Location

United States

Related Subject Headings

  • T-Lymphocyte Subsets
  • Receptors, Antigen, T-Cell, alpha-beta
  • Proteins
  • Oligonucleotide Array Sequence Analysis
  • Mice, Inbred C57BL
  • Mice
  • Immunology
  • Immunity
  • Gene Expression Regulation
  • Gene Expression
 

Citation

APA
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ICMJE
MLA
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Denning, T. L., Granger, S. W., Mucida, D., Graddy, R., Leclercq, G., Zhang, W., … Kronenberg, M. (2007). Mouse TCRalphabeta+CD8alphaalpha intraepithelial lymphocytes express genes that down-regulate their antigen reactivity and suppress immune responses. J Immunol, 178(7), 4230–4239. https://doi.org/10.4049/jimmunol.178.7.4230
Denning, Timothy L., Steve W. Granger, Daniel Mucida, Ryan Graddy, Georges Leclercq, Weiguo Zhang, Karen Honey, et al. “Mouse TCRalphabeta+CD8alphaalpha intraepithelial lymphocytes express genes that down-regulate their antigen reactivity and suppress immune responses.J Immunol 178, no. 7 (April 1, 2007): 4230–39. https://doi.org/10.4049/jimmunol.178.7.4230.
Denning TL, Granger SW, Mucida D, Graddy R, Leclercq G, Zhang W, et al. Mouse TCRalphabeta+CD8alphaalpha intraepithelial lymphocytes express genes that down-regulate their antigen reactivity and suppress immune responses. J Immunol. 2007 Apr 1;178(7):4230–9.
Denning, Timothy L., et al. “Mouse TCRalphabeta+CD8alphaalpha intraepithelial lymphocytes express genes that down-regulate their antigen reactivity and suppress immune responses.J Immunol, vol. 178, no. 7, Apr. 2007, pp. 4230–39. Pubmed, doi:10.4049/jimmunol.178.7.4230.
Denning TL, Granger SW, Mucida D, Graddy R, Leclercq G, Zhang W, Honey K, Rasmussen JP, Cheroutre H, Rudensky AY, Kronenberg M. Mouse TCRalphabeta+CD8alphaalpha intraepithelial lymphocytes express genes that down-regulate their antigen reactivity and suppress immune responses. J Immunol. 2007 Apr 1;178(7):4230–4239.

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

April 1, 2007

Volume

178

Issue

7

Start / End Page

4230 / 4239

Location

United States

Related Subject Headings

  • T-Lymphocyte Subsets
  • Receptors, Antigen, T-Cell, alpha-beta
  • Proteins
  • Oligonucleotide Array Sequence Analysis
  • Mice, Inbred C57BL
  • Mice
  • Immunology
  • Immunity
  • Gene Expression Regulation
  • Gene Expression