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Human cytidine triphosphate synthetase 1 interacting proteins.

Publication ,  Journal Article
Higgins, MJ; Loiselle, D; Haystead, TA; Graves, LM
Published in: Nucleosides Nucleotides Nucleic Acids
June 2008
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We investigated the interacting proteins and intracellular localization of CTP synthetase 1 (CTPS1) in mammalian cells. CTPS1 interacted with a GST- peptidyl prolyl isomerase, Pin1 fusion (GST-Pin1) in a Ser 575 (S575) phosphorylation-dependent manner. Immunoprecipitation experiments demonstrated that CTPS1 also bound tubulin, and thirteen additional coimmunoprecipitating proteins were identified by mass spectrometry. Immunolocalization experiments showed that tubulin and CTPS1 colocalized subcellularly. Taxol treatment enhanced this but cotreatment of cells with the CTPS inhibitor, cyclopentenyl cytosine (CPEC), and taxol failed to disrupt the colocalization. Thus, these studies provide novel information on the potential interacting proteins that may regulate CTPS1 function or intracellular localization.

Duke Scholars

Timothy Arthur James Haystead
Author Timothy Arthur James Haystead Pharmacology & Cancer Biology
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Published In

Nucleosides Nucleotides Nucleic Acids

DOI

10.1080/15257770802146502

EISSN

1532-2335

Publication Date

June 2008

Volume

27

Issue

6

Start / End Page

850 / 857

Location

United States

Related Subject Headings

  • Protein Binding
  • Peptidylprolyl Isomerase
  • Organic Chemistry
  • Mass Spectrometry
  • Immunoprecipitation
  • Humans
  • Cell Line
  • Carbon-Nitrogen Ligases
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
 

Citation

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Higgins, M. J., Loiselle, D., Haystead, T. A., & Graves, L. M. (2008). Human cytidine triphosphate synthetase 1 interacting proteins. Nucleosides Nucleotides Nucleic Acids, 27(6), 850–857. https://doi.org/10.1080/15257770802146502
Higgins, M. J., D. Loiselle, T. A. Haystead, and L. M. Graves. “Human cytidine triphosphate synthetase 1 interacting proteins.” Nucleosides Nucleotides Nucleic Acids 27, no. 6 (June 2008): 850–57. https://doi.org/10.1080/15257770802146502.
Higgins MJ, Loiselle D, Haystead TA, Graves LM. Human cytidine triphosphate synthetase 1 interacting proteins. Nucleosides Nucleotides Nucleic Acids. 2008 Jun;27(6):850–7.
Higgins, M. J., et al. “Human cytidine triphosphate synthetase 1 interacting proteins.” Nucleosides Nucleotides Nucleic Acids, vol. 27, no. 6, June 2008, pp. 850–57. Pubmed, doi:10.1080/15257770802146502.
Higgins MJ, Loiselle D, Haystead TA, Graves LM. Human cytidine triphosphate synthetase 1 interacting proteins. Nucleosides Nucleotides Nucleic Acids. 2008 Jun;27(6):850–857.

Published In

Nucleosides Nucleotides Nucleic Acids

DOI

10.1080/15257770802146502

EISSN

1532-2335

Publication Date

June 2008

Volume

27

Issue

6

Start / End Page

850 / 857

Location

United States

Related Subject Headings

  • Protein Binding
  • Peptidylprolyl Isomerase
  • Organic Chemistry
  • Mass Spectrometry
  • Immunoprecipitation
  • Humans
  • Cell Line
  • Carbon-Nitrogen Ligases
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology