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Composition and dynamics of the Caenorhabditis elegans early embryonic transcriptome.

Publication ,  Journal Article
Baugh, LR; Hill, AA; Slonim, DK; Brown, EL; Hunter, CP
Published in: Development (Cambridge, England)
March 2003

Temporal profiles of transcript abundance during embryonic development were obtained by whole-genome expression analysis from precisely staged C. elegans embryos. The result is a highly resolved time course that commences with the zygote and extends into mid-gastrulation, spanning the transition from maternal to embryonic control of development and including the presumptive specification of most major cell fates. Transcripts for nearly half (8890) of the predicted open reading frames are detected and expression levels for the majority of them (>70%) change over time. The transcriptome is stable up to the four-cell stage where it begins rapidly changing until the rate of change plateaus before gastrulation. At gastrulation temporal patterns of maternal degradation and embryonic expression intersect indicating a mid-blastula transition from maternal to embryonic control of development. In addition, we find that embryonic genes tend to be expressed transiently on a time scale consistent with developmental decisions being made with each cell cycle. Furthermore, overall rates of synthesis and degradation are matched such that the transcriptome maintains a steady-state frequency distribution. Finally, a versatile analytical platform based on cluster analysis and developmental classification of genes is provided.

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Published In

Development (Cambridge, England)

DOI

EISSN

1477-9129

ISSN

0950-1991

Publication Date

March 2003

Volume

130

Issue

5

Start / End Page

889 / 900

Related Subject Headings

  • Transcription, Genetic
  • Time Factors
  • RNA
  • Open Reading Frames
  • Oligonucleotide Array Sequence Analysis
  • Multigene Family
  • Genome
  • Gene Expression Regulation, Developmental
  • Gene Expression Profiling
  • Cell Lineage
 

Citation

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MLA
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Baugh, L. R., Hill, A. A., Slonim, D. K., Brown, E. L., & Hunter, C. P. (2003). Composition and dynamics of the Caenorhabditis elegans early embryonic transcriptome. Development (Cambridge, England), 130(5), 889–900. https://doi.org/10.1242/dev.00302
Baugh, L Ryan, Andrew A. Hill, Donna K. Slonim, Eugene L. Brown, and Craig P. Hunter. “Composition and dynamics of the Caenorhabditis elegans early embryonic transcriptome.Development (Cambridge, England) 130, no. 5 (March 2003): 889–900. https://doi.org/10.1242/dev.00302.
Baugh LR, Hill AA, Slonim DK, Brown EL, Hunter CP. Composition and dynamics of the Caenorhabditis elegans early embryonic transcriptome. Development (Cambridge, England). 2003 Mar;130(5):889–900.
Baugh, L. Ryan, et al. “Composition and dynamics of the Caenorhabditis elegans early embryonic transcriptome.Development (Cambridge, England), vol. 130, no. 5, Mar. 2003, pp. 889–900. Epmc, doi:10.1242/dev.00302.
Baugh LR, Hill AA, Slonim DK, Brown EL, Hunter CP. Composition and dynamics of the Caenorhabditis elegans early embryonic transcriptome. Development (Cambridge, England). 2003 Mar;130(5):889–900.
Journal cover image

Published In

Development (Cambridge, England)

DOI

EISSN

1477-9129

ISSN

0950-1991

Publication Date

March 2003

Volume

130

Issue

5

Start / End Page

889 / 900

Related Subject Headings

  • Transcription, Genetic
  • Time Factors
  • RNA
  • Open Reading Frames
  • Oligonucleotide Array Sequence Analysis
  • Multigene Family
  • Genome
  • Gene Expression Regulation, Developmental
  • Gene Expression Profiling
  • Cell Lineage