Structural evidence that the P/Q domain of ZipA is an unstructured, flexible tether between the membrane and the C-terminal FtsZ-binding domain.
Publication
, Journal Article
Ohashi, T; Hale, CA; de Boer, PAJ; Erickson, HP
Published in: J Bacteriol
August 2002
The cell division protein ZipA has an N-terminal transmembrane domain and a C-terminal globular domain that binds FtsZ. Between them are a charged domain and a P/Q domain rich in proline and glutamine that has been proposed to be an unfolded polypeptide. Here we provide evidence obtained by electron microscopy that the P/Q domain is a flexible tether ranging in length from 8 to 20 nm and invisible in rotary shadowing electron microscopy. We estimated a persistence length of 0.66 nm, which is similar to the persistence lengths of other unfolded and unstructured polypeptides.
Duke Scholars
Published In
J Bacteriol
DOI
ISSN
0021-9193
Publication Date
August 2002
Volume
184
Issue
15
Start / End Page
4313 / 4315
Location
United States
Related Subject Headings
- Protein Conformation
- Protein Binding
- Proline
- Models, Molecular
- Microscopy, Electron
- Microbiology
- Luminescent Proteins
- Green Fluorescent Proteins
- Glutamine
- Escherichia coli Proteins
Citation
APA
Chicago
ICMJE
MLA
NLM
Ohashi, T., Hale, C. A., de Boer, P. A. J., & Erickson, H. P. (2002). Structural evidence that the P/Q domain of ZipA is an unstructured, flexible tether between the membrane and the C-terminal FtsZ-binding domain. J Bacteriol, 184(15), 4313–4315. https://doi.org/10.1128/JB.184.15.4313-4315.2002
Ohashi, Tomoo, Cynthia A. Hale, Piet A. J. de Boer, and Harold P. Erickson. “Structural evidence that the P/Q domain of ZipA is an unstructured, flexible tether between the membrane and the C-terminal FtsZ-binding domain.” J Bacteriol 184, no. 15 (August 2002): 4313–15. https://doi.org/10.1128/JB.184.15.4313-4315.2002.
Ohashi T, Hale CA, de Boer PAJ, Erickson HP. Structural evidence that the P/Q domain of ZipA is an unstructured, flexible tether between the membrane and the C-terminal FtsZ-binding domain. J Bacteriol. 2002 Aug;184(15):4313–5.
Ohashi, Tomoo, et al. “Structural evidence that the P/Q domain of ZipA is an unstructured, flexible tether between the membrane and the C-terminal FtsZ-binding domain.” J Bacteriol, vol. 184, no. 15, Aug. 2002, pp. 4313–15. Pubmed, doi:10.1128/JB.184.15.4313-4315.2002.
Ohashi T, Hale CA, de Boer PAJ, Erickson HP. Structural evidence that the P/Q domain of ZipA is an unstructured, flexible tether between the membrane and the C-terminal FtsZ-binding domain. J Bacteriol. 2002 Aug;184(15):4313–4315.
Published In
J Bacteriol
DOI
ISSN
0021-9193
Publication Date
August 2002
Volume
184
Issue
15
Start / End Page
4313 / 4315
Location
United States
Related Subject Headings
- Protein Conformation
- Protein Binding
- Proline
- Models, Molecular
- Microscopy, Electron
- Microbiology
- Luminescent Proteins
- Green Fluorescent Proteins
- Glutamine
- Escherichia coli Proteins