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Structural evidence that the P/Q domain of ZipA is an unstructured, flexible tether between the membrane and the C-terminal FtsZ-binding domain.

Publication ,  Journal Article
Ohashi, T; Hale, CA; de Boer, PAJ; Erickson, HP
Published in: J Bacteriol
August 2002

The cell division protein ZipA has an N-terminal transmembrane domain and a C-terminal globular domain that binds FtsZ. Between them are a charged domain and a P/Q domain rich in proline and glutamine that has been proposed to be an unfolded polypeptide. Here we provide evidence obtained by electron microscopy that the P/Q domain is a flexible tether ranging in length from 8 to 20 nm and invisible in rotary shadowing electron microscopy. We estimated a persistence length of 0.66 nm, which is similar to the persistence lengths of other unfolded and unstructured polypeptides.

Duke Scholars

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

August 2002

Volume

184

Issue

15

Start / End Page

4313 / 4315

Location

United States

Related Subject Headings

  • Protein Conformation
  • Protein Binding
  • Proline
  • Models, Molecular
  • Microscopy, Electron
  • Microbiology
  • Luminescent Proteins
  • Green Fluorescent Proteins
  • Glutamine
  • Escherichia coli Proteins
 

Citation

APA
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ICMJE
MLA
NLM
Ohashi, T., Hale, C. A., de Boer, P. A. J., & Erickson, H. P. (2002). Structural evidence that the P/Q domain of ZipA is an unstructured, flexible tether between the membrane and the C-terminal FtsZ-binding domain. J Bacteriol, 184(15), 4313–4315. https://doi.org/10.1128/JB.184.15.4313-4315.2002
Ohashi, Tomoo, Cynthia A. Hale, Piet A. J. de Boer, and Harold P. Erickson. “Structural evidence that the P/Q domain of ZipA is an unstructured, flexible tether between the membrane and the C-terminal FtsZ-binding domain.J Bacteriol 184, no. 15 (August 2002): 4313–15. https://doi.org/10.1128/JB.184.15.4313-4315.2002.
Ohashi, Tomoo, et al. “Structural evidence that the P/Q domain of ZipA is an unstructured, flexible tether between the membrane and the C-terminal FtsZ-binding domain.J Bacteriol, vol. 184, no. 15, Aug. 2002, pp. 4313–15. Pubmed, doi:10.1128/JB.184.15.4313-4315.2002.

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

August 2002

Volume

184

Issue

15

Start / End Page

4313 / 4315

Location

United States

Related Subject Headings

  • Protein Conformation
  • Protein Binding
  • Proline
  • Models, Molecular
  • Microscopy, Electron
  • Microbiology
  • Luminescent Proteins
  • Green Fluorescent Proteins
  • Glutamine
  • Escherichia coli Proteins