Structural characteristics of the desmin protofilament.
Publication
, Journal Article
Milam, L; Erickson, HP
Published in: J Ultrastruct Res
November 1984
Biochemical investigations of intermediate filaments in soluble or partially assembled forms are often difficult to perform due to the unusual insolubility of most types of intermediate filaments. However, desmin is soluble in 10 mM Tris. The structure of partially soluble native desmin was studied by gel-filtration chromatography and electron microscopy. The lowest molecular weight species of soluble desmin is a flexible rod averaging 53 nm in length. Calculations of f/fmin values from a previously published sedimentation value allowed comparisons with other elongated proteins. These values and the dimensions obtained from electron microscopy suggest that the desmin protofilament contains three or four protein subunits.
Duke Scholars
Published In
J Ultrastruct Res
DOI
ISSN
0022-5320
Publication Date
November 1984
Volume
89
Issue
2
Start / End Page
179 / 186
Location
United States
Related Subject Headings
- Molecular Weight
- Microscopy, Electron
- Intermediate Filaments
- Desmin
- Cytoskeleton
- Chromatography, Gel
- Chickens
- Biophysics
- Animals
- 3101 Biochemistry and cell biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Milam, L., & Erickson, H. P. (1984). Structural characteristics of the desmin protofilament. J Ultrastruct Res, 89(2), 179–186. https://doi.org/10.1016/s0022-5320(84)80013-1
Milam, L., and H. P. Erickson. “Structural characteristics of the desmin protofilament.” J Ultrastruct Res 89, no. 2 (November 1984): 179–86. https://doi.org/10.1016/s0022-5320(84)80013-1.
Milam L, Erickson HP. Structural characteristics of the desmin protofilament. J Ultrastruct Res. 1984 Nov;89(2):179–86.
Milam, L., and H. P. Erickson. “Structural characteristics of the desmin protofilament.” J Ultrastruct Res, vol. 89, no. 2, Nov. 1984, pp. 179–86. Pubmed, doi:10.1016/s0022-5320(84)80013-1.
Milam L, Erickson HP. Structural characteristics of the desmin protofilament. J Ultrastruct Res. 1984 Nov;89(2):179–186.
Published In
J Ultrastruct Res
DOI
ISSN
0022-5320
Publication Date
November 1984
Volume
89
Issue
2
Start / End Page
179 / 186
Location
United States
Related Subject Headings
- Molecular Weight
- Microscopy, Electron
- Intermediate Filaments
- Desmin
- Cytoskeleton
- Chromatography, Gel
- Chickens
- Biophysics
- Animals
- 3101 Biochemistry and cell biology