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Multiple conformations of PEVK proteins detected by single-molecule techniques.

Publication ,  Journal Article
Li, H; Oberhauser, AF; Redick, SD; Carrion-Vazquez, M; Erickson, HP; Fernandez, JM
Published in: Proc Natl Acad Sci U S A
September 11, 2001

An important component of muscle elasticity is the PEVK region of titin, so named because of the preponderance of these amino acids. However, the PEVK region, similar to other elastomeric proteins, is thought to form a random coil and therefore its structure cannot be determined by standard techniques. Here we combine single-molecule electron microscopy and atomic force microscopy to examine the conformations of the human cardiac titin PEVK region. In contrast to a simple random coil, we have found that cardiac PEVK shows a wide range of elastic conformations with end-to-end distances ranging from 9 to 24 nm and persistence lengths from 0.4 to 2.5 nm. Individual PEVK molecules retained their distinctive elastic conformations through many stretch-relaxation cycles, consistent with the view that these PEVK conformers cannot be interconverted by force. The multiple elastic conformations of cardiac PEVK may result from varying degrees of proline isomerization. The single-molecule techniques demonstrated here may help elucidate the conformation of other proteins that lack a well-defined structure.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

September 11, 2001

Volume

98

Issue

19

Start / End Page

10682 / 10686

Location

United States

Related Subject Headings

  • Protein Kinases
  • Protein Folding
  • Protein Conformation
  • Peptides
  • Muscle Proteins
  • Humans
  • Connectin
 

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Li, H., Oberhauser, A. F., Redick, S. D., Carrion-Vazquez, M., Erickson, H. P., & Fernandez, J. M. (2001). Multiple conformations of PEVK proteins detected by single-molecule techniques. Proc Natl Acad Sci U S A, 98(19), 10682–10686. https://doi.org/10.1073/pnas.191189098
Li, H., A. F. Oberhauser, S. D. Redick, M. Carrion-Vazquez, H. P. Erickson, and J. M. Fernandez. “Multiple conformations of PEVK proteins detected by single-molecule techniques.Proc Natl Acad Sci U S A 98, no. 19 (September 11, 2001): 10682–86. https://doi.org/10.1073/pnas.191189098.
Li H, Oberhauser AF, Redick SD, Carrion-Vazquez M, Erickson HP, Fernandez JM. Multiple conformations of PEVK proteins detected by single-molecule techniques. Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10682–6.
Li, H., et al. “Multiple conformations of PEVK proteins detected by single-molecule techniques.Proc Natl Acad Sci U S A, vol. 98, no. 19, Sept. 2001, pp. 10682–86. Pubmed, doi:10.1073/pnas.191189098.
Li H, Oberhauser AF, Redick SD, Carrion-Vazquez M, Erickson HP, Fernandez JM. Multiple conformations of PEVK proteins detected by single-molecule techniques. Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10682–10686.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

September 11, 2001

Volume

98

Issue

19

Start / End Page

10682 / 10686

Location

United States

Related Subject Headings

  • Protein Kinases
  • Protein Folding
  • Protein Conformation
  • Peptides
  • Muscle Proteins
  • Humans
  • Connectin