Enhancing and confirming the specificity of RNAi experiments.
Publication
, Journal Article
Cullen, BR
Published in: Nature methods
September 2006
RNA interference (RNAi) provides a powerful technique for the derivation and analysis of loss-of-function phenotypes in vertebrate cells, where alternative approaches are either arduous or frequently ineffective. RNAi, however, is not always totally specific, and confirming the specificity, and hence the validity, of data obtained using RNAi therefore forms an essential component of experiments that rely on this technique. Here I briefly review the potential pitfalls associated with RNAi, and then suggest experimental approaches that can be used to maximize the specificity of RNAi or to confirm that the data obtained are indeed valid.
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Published In
Nature methods
DOI
EISSN
1548-7105
ISSN
1548-7091
Publication Date
September 2006
Volume
3
Issue
9
Start / End Page
677 / 681
Related Subject Headings
- Sensitivity and Specificity
- RNA, Small Interfering
- RNA Interference
- Humans
- Gene Silencing
- Developmental Biology
- Animals
- 31 Biological sciences
- 11 Medical and Health Sciences
- 10 Technology
Citation
APA
Chicago
ICMJE
MLA
NLM
Cullen, B. R. (2006). Enhancing and confirming the specificity of RNAi experiments. Nature Methods, 3(9), 677–681. https://doi.org/10.1038/nmeth913
Cullen, Bryan R. “Enhancing and confirming the specificity of RNAi experiments.” Nature Methods 3, no. 9 (September 2006): 677–81. https://doi.org/10.1038/nmeth913.
Cullen BR. Enhancing and confirming the specificity of RNAi experiments. Nature methods. 2006 Sep;3(9):677–81.
Cullen, Bryan R. “Enhancing and confirming the specificity of RNAi experiments.” Nature Methods, vol. 3, no. 9, Sept. 2006, pp. 677–81. Epmc, doi:10.1038/nmeth913.
Cullen BR. Enhancing and confirming the specificity of RNAi experiments. Nature methods. 2006 Sep;3(9):677–681.
Published In
Nature methods
DOI
EISSN
1548-7105
ISSN
1548-7091
Publication Date
September 2006
Volume
3
Issue
9
Start / End Page
677 / 681
Related Subject Headings
- Sensitivity and Specificity
- RNA, Small Interfering
- RNA Interference
- Humans
- Gene Silencing
- Developmental Biology
- Animals
- 31 Biological sciences
- 11 Medical and Health Sciences
- 10 Technology