ApoE isoform affects LTP in human targeted replacement mice.
Inheritance of the epsilon4 allele for apolipoprotein E (apoE) increases the risk of Alzheimer disease and memory impairment, whereas epsilon2 decreases these risks compared with the most common epsilon3 allele, but the mechanism for these effects is unknown. Long-term potentiation (LTP) is an experimentally induced increase in synaptic efficacy that models memory. Using hippocampal slices from wild type (WT), apoE knockout (apoE-KO), and targeted replacement mice expressing human apoE2, E3, or E4 (apoE-TR) we found that although all strains had comparable basal synaptic transmission, LTP was significantly greater in WT and apoE3-TR than in apoE-KO, apoE2-TR or apoE4-TR. This novel system may be used to investigate the mechanisms of apoE isoform dependent modulation of susceptibility to memory impairment.
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Related Subject Headings
- Protein Isoforms
- Neurology & Neurosurgery
- Mice, Transgenic
- Mice, Inbred C57BL
- Mice
- Long-Term Potentiation
- In Vitro Techniques
- Humans
- Hippocampus
- Electric Stimulation
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Protein Isoforms
- Neurology & Neurosurgery
- Mice, Transgenic
- Mice, Inbred C57BL
- Mice
- Long-Term Potentiation
- In Vitro Techniques
- Humans
- Hippocampus
- Electric Stimulation