Skip to main content

Mutational analysis of the leucine zipper-like motif of the human immunodeficiency virus type 1 envelope transmembrane glycoprotein.

Publication ,  Journal Article
Chen, SS; Lee, CN; Lee, WR; McIntosh, K; Lee, TH
Published in: J Virol
June 1993

The N-terminal region of the envelope (env) transmembrane protein of human immunodeficiency virus type 1 (HIV-1) has a leucine zipper-like motif. This highly conserved zipper motif, which consists of a heptad repeat of leucine or isoleucine residues, has been suggested to play a role in HIV-1 env glycoprotein oligomerization. This hypothesis was tested by replacing the highly conserved leucine or isoleucine residues in the zipper motif with a strong alpha-helix breaker, proline. We report here that such substitutions did not abolish the ability of env protein to form oligomers, indicating that this highly conserved zipper motif does not have a crucial role in env protein oligomerization. However, the mutant viruses all showed impaired infectivity, suggesting that this conserved zipper motif can have an important role in the virus life cycle.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

June 1993

Volume

67

Issue

6

Start / End Page

3615 / 3619

Location

United States

Related Subject Headings

  • Virology
  • Protein Structure, Secondary
  • Protein Conformation
  • Proline
  • Molecular Sequence Data
  • Leucine Zippers
  • Leucine
  • Isoleucine
  • HIV-1
  • HIV Envelope Protein gp41
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Chen, S. S., Lee, C. N., Lee, W. R., McIntosh, K., & Lee, T. H. (1993). Mutational analysis of the leucine zipper-like motif of the human immunodeficiency virus type 1 envelope transmembrane glycoprotein. J Virol, 67(6), 3615–3619. https://doi.org/10.1128/JVI.67.6.3615-3619.1993
Chen, S. S., C. N. Lee, W. R. Lee, K. McIntosh, and T. H. Lee. “Mutational analysis of the leucine zipper-like motif of the human immunodeficiency virus type 1 envelope transmembrane glycoprotein.J Virol 67, no. 6 (June 1993): 3615–19. https://doi.org/10.1128/JVI.67.6.3615-3619.1993.
Chen, S. S., et al. “Mutational analysis of the leucine zipper-like motif of the human immunodeficiency virus type 1 envelope transmembrane glycoprotein.J Virol, vol. 67, no. 6, June 1993, pp. 3615–19. Pubmed, doi:10.1128/JVI.67.6.3615-3619.1993.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

June 1993

Volume

67

Issue

6

Start / End Page

3615 / 3619

Location

United States

Related Subject Headings

  • Virology
  • Protein Structure, Secondary
  • Protein Conformation
  • Proline
  • Molecular Sequence Data
  • Leucine Zippers
  • Leucine
  • Isoleucine
  • HIV-1
  • HIV Envelope Protein gp41