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Interaction of calcium and lead in human erythrocytes.

Publication ,  Journal Article
Ong, CN; Lee, WR
Published in: British journal of industrial medicine
February 1980

The interactions of calcium and lead on the human erythrocytes have been studied in vitro using 45Ca and 203Pb as tracers. The chemical groups binding calcium and lead on the erythrocytes were also investigated. Calcium ions in the plasma were shown to be capable of replacing the 203Pb on the red cells. More than 85% of the 203Pb in the erythrocyte was associated with the cytoplasmic components, and the rest was bound to the stromal membrane. About 90% of 45Ca was attached to erythrocyte membrane. Extraction of 45Ca and 203Pb-labelled erythrocyte membranes with chloroform/methanol mixture showed that the distribution patterns of these two nuclides are similar, with over 88% protein bound, less than 10% lipid bound, and traces in the aqueous phase. Chemical modification of erythrocyte membrane proteins with carbodi-imide, p-chloromercuribenzoate (PCMB), and maleic anhydride suggested that the carboxyl groups are responsible for binding lead and calcium to the red cell membrane. The SH groups may have a minor role in the binding for both cations. Amino groups did not appear to affect the binding of these cations. Gel chromatography of 45Ca-labelled erythrocyte membrane indicated that Ca++ bound to the same fraction of membrane proteins as 203Pb, corresponding to a molecular weight of about 130 000 to 230 000. A possible implication of these findings is that lead and calcium may compete for the same binding site(s) on the erythrocyte.

Published In

British journal of industrial medicine

ISSN

0007-1072

Publication Date

February 1980

Volume

37

Issue

1

Start / End Page

70 / 77

Location

england

Related Subject Headings

  • Maleic Anhydrides
  • Lead
  • In Vitro Techniques
  • Humans
  • Erythrocytes
  • Erythrocyte Membrane
  • Cyanamide
  • Chromatography, Gel
  • Chloromercuribenzoates
  • Calcium
 

Citation

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Ong, C. N., & Lee, W. R. (1980). Interaction of calcium and lead in human erythrocytes. British Journal of Industrial Medicine, 37(1), 70–77.
Ong, C. N., and W. R. Lee. “Interaction of calcium and lead in human erythrocytes.British Journal of Industrial Medicine 37, no. 1 (February 1980): 70–77.
Ong CN, Lee WR. Interaction of calcium and lead in human erythrocytes. British journal of industrial medicine. 1980 Feb;37(1):70–7.
Ong, C. N., and W. R. Lee. “Interaction of calcium and lead in human erythrocytes.British Journal of Industrial Medicine, vol. 37, no. 1, Feb. 1980, pp. 70–77.
Ong CN, Lee WR. Interaction of calcium and lead in human erythrocytes. British journal of industrial medicine. 1980 Feb;37(1):70–77.

Published In

British journal of industrial medicine

ISSN

0007-1072

Publication Date

February 1980

Volume

37

Issue

1

Start / End Page

70 / 77

Location

england

Related Subject Headings

  • Maleic Anhydrides
  • Lead
  • In Vitro Techniques
  • Humans
  • Erythrocytes
  • Erythrocyte Membrane
  • Cyanamide
  • Chromatography, Gel
  • Chloromercuribenzoates
  • Calcium