Immunogold fine structural localization of extracellular matrix components in aged human cornea. I. Types I-IV collagen and laminin.
Publication
, Journal Article
Marshall, GE; Konstas, AG; Lee, WR
Published in: Graefes Arch Clin Exp Ophthalmol
1991
Using the immunogold technique combined with cryoultramicrotomy and London Resin white (LR white) embedding, we studied the fine structural distribution of types I-IV collagen and laminin in corneal tissue from seven enucleated human eyes (age range, 63-78 years). Type II collagen was not identified in any corneal layer. Type I and type III collagen were distributed in a similar fashion in striated collagen fibrils in Bowman's layer and in the stroma. Type IV collagen was located only in the posterior non-banded region of Descemet's membrane. Laminin was identified in subepithelial anchoring plaques and the sub-endothelial region of Descemet's membrane in accordance with its recognized adhesive function.
Duke Scholars
Published In
Graefes Arch Clin Exp Ophthalmol
DOI
ISSN
0721-832X
Publication Date
1991
Volume
229
Issue
2
Start / End Page
157 / 163
Location
Germany
Related Subject Headings
- Ophthalmology & Optometry
- Middle Aged
- Male
- Laminin
- Immunohistochemistry
- Humans
- Female
- Extracellular Matrix Proteins
- Cornea
- Collagen
Citation
APA
Chicago
ICMJE
MLA
NLM
Marshall, G. E., Konstas, A. G., & Lee, W. R. (1991). Immunogold fine structural localization of extracellular matrix components in aged human cornea. I. Types I-IV collagen and laminin. Graefes Arch Clin Exp Ophthalmol, 229(2), 157–163. https://doi.org/10.1007/BF00170550
Marshall, G. E., A. G. Konstas, and W. R. Lee. “Immunogold fine structural localization of extracellular matrix components in aged human cornea. I. Types I-IV collagen and laminin.” Graefes Arch Clin Exp Ophthalmol 229, no. 2 (1991): 157–63. https://doi.org/10.1007/BF00170550.
Marshall GE, Konstas AG, Lee WR. Immunogold fine structural localization of extracellular matrix components in aged human cornea. I. Types I-IV collagen and laminin. Graefes Arch Clin Exp Ophthalmol. 1991;229(2):157–63.
Marshall, G. E., et al. “Immunogold fine structural localization of extracellular matrix components in aged human cornea. I. Types I-IV collagen and laminin.” Graefes Arch Clin Exp Ophthalmol, vol. 229, no. 2, 1991, pp. 157–63. Pubmed, doi:10.1007/BF00170550.
Marshall GE, Konstas AG, Lee WR. Immunogold fine structural localization of extracellular matrix components in aged human cornea. I. Types I-IV collagen and laminin. Graefes Arch Clin Exp Ophthalmol. 1991;229(2):157–163.
Published In
Graefes Arch Clin Exp Ophthalmol
DOI
ISSN
0721-832X
Publication Date
1991
Volume
229
Issue
2
Start / End Page
157 / 163
Location
Germany
Related Subject Headings
- Ophthalmology & Optometry
- Middle Aged
- Male
- Laminin
- Immunohistochemistry
- Humans
- Female
- Extracellular Matrix Proteins
- Cornea
- Collagen