Addition of molecular methods to mutation studies with Drosophila melanogaster.
For 80 years, Drosophila melanogaster has been used as a major tool in analyzing Mendelian genetics. By using chromosome inversions that suppress crossing over, geneticists have developed a large number of stocks for mutation analysis. These stocks permit numerous tests for specific locus mutations, lethals at multiple loci on any chromosome, chromosome exchanges, insertions, and deletions. The entire genome can be manipulated for a degree of genetic control not found in other germ-line systems. Recombinant DNA techniques now permit analysis of mutations to the nucleotide level. By combining classical genetic analysis with recombinant DNA techniques, it is possible to analyze mutations that range from chromosome aberrations and multilocus deficiencies to single nucleotide transitions.
Duke Scholars
Published In
DOI
ISSN
Publication Date
Volume
Start / End Page
Location
Related Subject Headings
- Toxicology
- Drosophila melanogaster
- DNA, Recombinant
- DNA Mutational Analysis
- Base Sequence
- Animals
- 4206 Public health
- 3206 Medical biotechnology
- 3105 Genetics
- 11 Medical and Health Sciences
Citation
Published In
DOI
ISSN
Publication Date
Volume
Start / End Page
Location
Related Subject Headings
- Toxicology
- Drosophila melanogaster
- DNA, Recombinant
- DNA Mutational Analysis
- Base Sequence
- Animals
- 4206 Public health
- 3206 Medical biotechnology
- 3105 Genetics
- 11 Medical and Health Sciences