Monoclonal antibodies to human primary uveal melanomas demonstrate tumor heterogeneity.
Publication
, Journal Article
Damato, BE; Campbell, AM; McGuire, BJ; Lee, WR; Foulds, WS
Published in: Invest Ophthalmol Vis Sci
September 1986
Five rat monoclonal antibodies to human uveal melanoma were produced using primary tumor tissue for immunization and screening. These monoclonal antibodies were tested by enzyme-linked immunosorbent assay against 12 uveal melanomas, one breast carcinoma, and peripheral blood lymphocytes from five healthy volunteers. One monoclonal antibody, 4A3, reacted against all the melanomas and none of the controls. On immunoblotting, this monoclonal antibody identified a protein doublet in the molecular weight range 55,000. With these monoclonal antibodies, it was possible to demonstrate significant variation in the antigenic profiles of the uveal melanomas which was not present in the controls.
Duke Scholars
Published In
Invest Ophthalmol Vis Sci
ISSN
0146-0404
Publication Date
September 1986
Volume
27
Issue
9
Start / End Page
1362 / 1367
Location
United States
Related Subject Headings
- Uveal Neoplasms
- Rats, Inbred Strains
- Rats
- Ophthalmology & Optometry
- Melanoma
- Humans
- Enzyme-Linked Immunosorbent Assay
- Antigens, Neoplasm
- Antibodies, Monoclonal
- Animals
Citation
APA
Chicago
ICMJE
MLA
NLM
Damato, B. E., Campbell, A. M., McGuire, B. J., Lee, W. R., & Foulds, W. S. (1986). Monoclonal antibodies to human primary uveal melanomas demonstrate tumor heterogeneity. Invest Ophthalmol Vis Sci, 27(9), 1362–1367.
Damato, B. E., A. M. Campbell, B. J. McGuire, W. R. Lee, and W. S. Foulds. “Monoclonal antibodies to human primary uveal melanomas demonstrate tumor heterogeneity.” Invest Ophthalmol Vis Sci 27, no. 9 (September 1986): 1362–67.
Damato BE, Campbell AM, McGuire BJ, Lee WR, Foulds WS. Monoclonal antibodies to human primary uveal melanomas demonstrate tumor heterogeneity. Invest Ophthalmol Vis Sci. 1986 Sep;27(9):1362–7.
Damato, B. E., et al. “Monoclonal antibodies to human primary uveal melanomas demonstrate tumor heterogeneity.” Invest Ophthalmol Vis Sci, vol. 27, no. 9, Sept. 1986, pp. 1362–67.
Damato BE, Campbell AM, McGuire BJ, Lee WR, Foulds WS. Monoclonal antibodies to human primary uveal melanomas demonstrate tumor heterogeneity. Invest Ophthalmol Vis Sci. 1986 Sep;27(9):1362–1367.
Published In
Invest Ophthalmol Vis Sci
ISSN
0146-0404
Publication Date
September 1986
Volume
27
Issue
9
Start / End Page
1362 / 1367
Location
United States
Related Subject Headings
- Uveal Neoplasms
- Rats, Inbred Strains
- Rats
- Ophthalmology & Optometry
- Melanoma
- Humans
- Enzyme-Linked Immunosorbent Assay
- Antigens, Neoplasm
- Antibodies, Monoclonal
- Animals