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Caenorhabditis elegans-based screen identifies Salmonella virulence factors required for conserved host-pathogen interactions.

Publication ,  Journal Article
Tenor, JL; McCormick, BA; Ausubel, FM; Aballay, A
Published in: Curr Biol
June 8, 2004

A Caenorhabditis elegans-Salmonella enterica host-pathogen model was used to identify both novel and previously known S. enterica virulence factors (HilA, HilD, InvH, SptP, RhuM, Spi4-F, PipA, VsdA, RepC, Sb25, RfaL, GmhA, LeuO, CstA, and RecC), including several related to the type III secretion system (TTSS) encoded in Salmonella pathogenicity island 1 (SPI-1). Mutants corresponding to presumptive novel virulence-related genes exhibited diminished ability to invade epithelial cells and/or to induce polymorphonuclear leukocyte migration in a tissue culture model of mammalian enteropathogenesis. When expressed in C. elegans intestinal cells, the S. enterica TTSS-exported effector protein SptP inhibited a conserved p38 MAPK signaling pathway and suppressed the diminished pathogenicity phenotype of an S. enterica sptP mutant. These results show that C. elegans is an attractive model to study the interaction between Salmonella effector proteins and components of the innate immune response, in part because there is a remarkable overlap between Salmonella virulence factors required for human and nematode pathogenesis.

Duke Scholars

Published In

Curr Biol

DOI

ISSN

0960-9822

Publication Date

June 8, 2004

Volume

14

Issue

11

Start / End Page

1018 / 1024

Location

England

Related Subject Headings

  • p38 Mitogen-Activated Protein Kinases
  • Virulence Factors
  • Virulence
  • Time Factors
  • Temperature
  • Salmonella enterica
  • Salmonella Infections
  • Phenotype
  • Mutation
  • Mitogen-Activated Protein Kinases
 

Citation

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Tenor, J. L., McCormick, B. A., Ausubel, F. M., & Aballay, A. (2004). Caenorhabditis elegans-based screen identifies Salmonella virulence factors required for conserved host-pathogen interactions. Curr Biol, 14(11), 1018–1024. https://doi.org/10.1016/j.cub.2004.05.050
Tenor, Jennifer L., Beth A. McCormick, Frederick M. Ausubel, and Alejandro Aballay. “Caenorhabditis elegans-based screen identifies Salmonella virulence factors required for conserved host-pathogen interactions.Curr Biol 14, no. 11 (June 8, 2004): 1018–24. https://doi.org/10.1016/j.cub.2004.05.050.
Tenor JL, McCormick BA, Ausubel FM, Aballay A. Caenorhabditis elegans-based screen identifies Salmonella virulence factors required for conserved host-pathogen interactions. Curr Biol. 2004 Jun 8;14(11):1018–24.
Tenor, Jennifer L., et al. “Caenorhabditis elegans-based screen identifies Salmonella virulence factors required for conserved host-pathogen interactions.Curr Biol, vol. 14, no. 11, June 2004, pp. 1018–24. Pubmed, doi:10.1016/j.cub.2004.05.050.
Tenor JL, McCormick BA, Ausubel FM, Aballay A. Caenorhabditis elegans-based screen identifies Salmonella virulence factors required for conserved host-pathogen interactions. Curr Biol. 2004 Jun 8;14(11):1018–1024.
Journal cover image

Published In

Curr Biol

DOI

ISSN

0960-9822

Publication Date

June 8, 2004

Volume

14

Issue

11

Start / End Page

1018 / 1024

Location

England

Related Subject Headings

  • p38 Mitogen-Activated Protein Kinases
  • Virulence Factors
  • Virulence
  • Time Factors
  • Temperature
  • Salmonella enterica
  • Salmonella Infections
  • Phenotype
  • Mutation
  • Mitogen-Activated Protein Kinases