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Stress affects uterine receptivity through an ovarian-independent pathway.

Publication ,  Journal Article
Kondoh, E; Okamoto, T; Higuchi, T; Tatsumi, K; Baba, T; Murphy, SK; Takakura, K; Konishi, I; Fujii, S
Published in: Hum Reprod
April 2009

BACKGROUND: Although stress is known to disturb natural fertility through the inhibition of the hypothalamic-pituitary-gonadal (HPG) axis, the impact of stress on infertile women who receive exogenous gonadal hormones is not well defined. This is probably due to lack of experimental models for evaluating the impacts of stress through an ovarian-independent pathway. The objective of this study was to investigate the possible impact of stress on uterine receptivity, independent of HPG axis dysfunction, using a mouse implantation model maintained with hormone supplementation. METHODS: Blastocysts from donor mice were transferred into the uterine lumen of ovariectomized (OVX) Balb/c female recipient mice following supplementation with estradiol and progesterone. The recipients were divided into two groups: those exposed (stress group) or not exposed (control group) to intermittent sonic exposure prior to embryo transfer (ET). The number of implantation sites (IS) was compared between these groups. Microarray analysis was performed to elucidate stress-induced molecular alterations in uteri during the implantation period. Sequential gene expression of leukemia inhibitory factor (Lif), an estradiol-inducible gene, was also analyzed using real-time PCR. RESULTS: A non-mating OVX model with satisfactory implantation rates was established. The number of IS in the stress group (n = 20) was significantly less than that in the control group (n = 18) (Mann-Whitney test, P = 0.0375). Implantation-related genes and ovarian-hormone-responsive genes were repressed in the stress group despite ovarian hormone supplementation. The expression of Lif was suppressed in the stress group. CONCLUSIONS: Stress can cause decreased uterine receptivity through an ovarian-independent pathway.

Duke Scholars

Published In

Hum Reprod

DOI

EISSN

1460-2350

Publication Date

April 2009

Volume

24

Issue

4

Start / End Page

945 / 953

Location

England

Related Subject Headings

  • Uterus
  • Stress, Physiological
  • Progesterone
  • Pregnancy
  • Ovary
  • Ovariectomy
  • Oligonucleotide Array Sequence Analysis
  • Obstetrics & Reproductive Medicine
  • Noise
  • Models, Animal
 

Citation

APA
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ICMJE
MLA
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Kondoh, E., Okamoto, T., Higuchi, T., Tatsumi, K., Baba, T., Murphy, S. K., … Fujii, S. (2009). Stress affects uterine receptivity through an ovarian-independent pathway. Hum Reprod, 24(4), 945–953. https://doi.org/10.1093/humrep/den461
Kondoh, Eiji, Takako Okamoto, Toshihiro Higuchi, Keiji Tatsumi, Tsukasa Baba, Susan K. Murphy, Kenji Takakura, Ikuo Konishi, and Shingo Fujii. “Stress affects uterine receptivity through an ovarian-independent pathway.Hum Reprod 24, no. 4 (April 2009): 945–53. https://doi.org/10.1093/humrep/den461.
Kondoh E, Okamoto T, Higuchi T, Tatsumi K, Baba T, Murphy SK, et al. Stress affects uterine receptivity through an ovarian-independent pathway. Hum Reprod. 2009 Apr;24(4):945–53.
Kondoh, Eiji, et al. “Stress affects uterine receptivity through an ovarian-independent pathway.Hum Reprod, vol. 24, no. 4, Apr. 2009, pp. 945–53. Pubmed, doi:10.1093/humrep/den461.
Kondoh E, Okamoto T, Higuchi T, Tatsumi K, Baba T, Murphy SK, Takakura K, Konishi I, Fujii S. Stress affects uterine receptivity through an ovarian-independent pathway. Hum Reprod. 2009 Apr;24(4):945–953.
Journal cover image

Published In

Hum Reprod

DOI

EISSN

1460-2350

Publication Date

April 2009

Volume

24

Issue

4

Start / End Page

945 / 953

Location

England

Related Subject Headings

  • Uterus
  • Stress, Physiological
  • Progesterone
  • Pregnancy
  • Ovary
  • Ovariectomy
  • Oligonucleotide Array Sequence Analysis
  • Obstetrics & Reproductive Medicine
  • Noise
  • Models, Animal