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Helicobacter infection decreases reproductive performance of IL10-deficient mice.

Publication ,  Journal Article
Sharp, JM; Vanderford, DA; Chichlowski, M; Myles, MH; Hale, LP
Published in: Comp Med
October 2008

Infections with a variety of Helicobacter species have been documented in rodent research facilities, with variable effects on rodent health. Helicobacter typhlonius has been reported to cause enteric disease in immunodeficient and IL10(-/-) mice, whereas H. rodentium has only been reported to cause disease in immunodeficient mice coinfected with other Helicobacter species. The effect of Helicobacter infections on murine reproduction has not been well studied. The reproductive performance of C57BL/6 IL10(-/-) female mice intentionally infected with H. typhlonius, H. rodentium, or both was compared with that of age-matched uninfected controls or similarly infected mice that received antihelicobacter therapy. The presence of Helicobacter organisms in stool and relevant tissues was detected by PCR assays. Helicobacter infection of IL10(-/-) female mice markedly decreased pregnancy rates and pup survival. The number of pups surviving to weaning was greatest in noninfected mice and decreased for H. rodentium > H. typhlonius >> H. rodentium and H. typhlonius coinfected mice. Helicobacter organisms were detected by semiquantitative real-time PCR in the reproductive organs of a subset of infected mice. Treatment of infected mice with a 4-drug regimen consisting of amoxicillin, clarithromycin, metronidazole, and omeprazole increased pregnancy rates, and pup survival and dam fecundity improved. We conclude that infection with H. typhlonius, H. rodentium, or both decreased the reproductive performance of IL10(-/-) mice. In addition, antihelicobacter therapy improved fecundity and enhanced pup survival.

Duke Scholars

Published In

Comp Med

ISSN

1532-0820

Publication Date

October 2008

Volume

58

Issue

5

Start / End Page

447 / 453

Location

United States

Related Subject Headings

  • Veterinary Sciences
  • Uterus
  • Rodent Diseases
  • Reproduction
  • Pregnancy
  • Ovary
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Male
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Sharp, J. M., Vanderford, D. A., Chichlowski, M., Myles, M. H., & Hale, L. P. (2008). Helicobacter infection decreases reproductive performance of IL10-deficient mice. Comp Med, 58(5), 447–453.
Sharp, Julie M., Deborah A. Vanderford, Maciej Chichlowski, Matthew H. Myles, and Laura P. Hale. “Helicobacter infection decreases reproductive performance of IL10-deficient mice.Comp Med 58, no. 5 (October 2008): 447–53.
Sharp JM, Vanderford DA, Chichlowski M, Myles MH, Hale LP. Helicobacter infection decreases reproductive performance of IL10-deficient mice. Comp Med. 2008 Oct;58(5):447–53.
Sharp, Julie M., et al. “Helicobacter infection decreases reproductive performance of IL10-deficient mice.Comp Med, vol. 58, no. 5, Oct. 2008, pp. 447–53.
Sharp JM, Vanderford DA, Chichlowski M, Myles MH, Hale LP. Helicobacter infection decreases reproductive performance of IL10-deficient mice. Comp Med. 2008 Oct;58(5):447–453.

Published In

Comp Med

ISSN

1532-0820

Publication Date

October 2008

Volume

58

Issue

5

Start / End Page

447 / 453

Location

United States

Related Subject Headings

  • Veterinary Sciences
  • Uterus
  • Rodent Diseases
  • Reproduction
  • Pregnancy
  • Ovary
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Male