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Aquaporin 6 binds calmodulin in a calcium-dependent manner.

Publication ,  Journal Article
Rabaud, NE; Song, L; Wang, Y; Agre, P; Yasui, M; Carbrey, JM
Published in: Biochem Biophys Res Commun
May 22, 2009
Published version (DOI) Link to item

Aquaporin 6 (AQP6) is an anion channel that is expressed primarily in acid secreting alpha-intercalated cells of the kidney collecting duct. In addition, AQP6 anion channel permeability is gated by low pH. Inspection of the N-terminus of AQP6 revealed a putative calmodulin binding site. AQP6-expressing CHO-K1 cell lysates were mixed with calmodulin beads and AQP6 was pulled down in the presence of calcium. Mutagenesis of the N-terminal calmodulin binding site in full length mouse AQP6 resulted in a loss of calmodulin binding activity. Mouse and human AQP6 calmodulin binding site peptides bound dansyl-calmodulin with a dissociation constant of approximately 1microM. The binding of AQP6 to calmodulin may be an important key to determining the physiological role of AQP6 in the kidney.

Duke Scholars

Jennifer Mehlman Carbrey
Author Jennifer Mehlman Carbrey Cell Biology

Published In

Biochem Biophys Res Commun

DOI

10.1016/j.bbrc.2009.03.128

EISSN

1090-2104

Publication Date

May 22, 2009

Volume

383

Issue

1

Start / End Page

54 / 57

Location

United States

Related Subject Headings

  • Rats
  • Protein Structure, Tertiary
  • Protein Binding
  • Molecular Sequence Data
  • Mice
  • Humans
  • Cricetulus
  • Cricetinae
  • Calmodulin
  • Calcium
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Rabaud, N. E., Song, L., Wang, Y., Agre, P., Yasui, M., & Carbrey, J. M. (2009). Aquaporin 6 binds calmodulin in a calcium-dependent manner. Biochem Biophys Res Commun, 383(1), 54–57. https://doi.org/10.1016/j.bbrc.2009.03.128
Rabaud, Nicole E., Linhua Song, Yiding Wang, Peter Agre, Masato Yasui, and Jennifer M. Carbrey. “Aquaporin 6 binds calmodulin in a calcium-dependent manner.” Biochem Biophys Res Commun 383, no. 1 (May 22, 2009): 54–57. https://doi.org/10.1016/j.bbrc.2009.03.128.
Rabaud NE, Song L, Wang Y, Agre P, Yasui M, Carbrey JM. Aquaporin 6 binds calmodulin in a calcium-dependent manner. Biochem Biophys Res Commun. 2009 May 22;383(1):54–7.
Rabaud, Nicole E., et al. “Aquaporin 6 binds calmodulin in a calcium-dependent manner.” Biochem Biophys Res Commun, vol. 383, no. 1, May 2009, pp. 54–57. Pubmed, doi:10.1016/j.bbrc.2009.03.128.
Rabaud NE, Song L, Wang Y, Agre P, Yasui M, Carbrey JM. Aquaporin 6 binds calmodulin in a calcium-dependent manner. Biochem Biophys Res Commun. 2009 May 22;383(1):54–57.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

10.1016/j.bbrc.2009.03.128

EISSN

1090-2104

Publication Date

May 22, 2009

Volume

383

Issue

1

Start / End Page

54 / 57

Location

United States

Related Subject Headings

  • Rats
  • Protein Structure, Tertiary
  • Protein Binding
  • Molecular Sequence Data
  • Mice
  • Humans
  • Cricetulus
  • Cricetinae
  • Calmodulin
  • Calcium