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Proteasome-mediated turnover of the transcription coactivator NPR1 plays dual roles in regulating plant immunity.

Publication ,  Journal Article
Spoel, SH; Mou, Z; Tada, Y; Spivey, NW; Genschik, P; Dong, X
Published in: Cell
May 2009

Systemic acquired resistance (SAR) is a broad-spectrum plant immune response involving profound transcriptional changes that are regulated by the coactivator NPR1. Nuclear translocation of NPR1 is a critical regulatory step, but how the protein is regulated in the nucleus is unknown. Here, we show that turnover of nuclear NPR1 protein plays an important role in modulating transcription of its target genes. In the absence of pathogen challenge, NPR1 is continuously cleared from the nucleus by the proteasome, which restricts its coactivator activity to prevent untimely activation of SAR. Surprisingly, inducers of SAR promote NPR1 phosphorylation at residues Ser11/Ser15, and then facilitate its recruitment to a Cullin3-based ubiquitin ligase. Turnover of phosphorylated NPR1 is required for full induction of target genes and establishment of SAR. These in vivo data demonstrate dual roles for coactivator turnover in both preventing and stimulating gene transcription to regulate plant immunity.

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Published In

Cell

DOI

EISSN

1097-4172

ISSN

0092-8674

Publication Date

May 2009

Volume

137

Issue

5

Start / End Page

860 / 872

Related Subject Headings

  • Transcription, Genetic
  • Proteasome Endopeptidase Complex
  • Phosphorylation
  • Immunity, Innate
  • Gene Expression Regulation, Plant
  • Developmental Biology
  • Cullin Proteins
  • Cell Nucleus
  • Carrier Proteins
  • Arabidopsis Proteins
 

Citation

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Spoel, S. H., Mou, Z., Tada, Y., Spivey, N. W., Genschik, P., & Dong, X. (2009). Proteasome-mediated turnover of the transcription coactivator NPR1 plays dual roles in regulating plant immunity. Cell, 137(5), 860–872. https://doi.org/10.1016/j.cell.2009.03.038
Spoel, Steven H., Zhonglin Mou, Yasuomi Tada, Natalie W. Spivey, Pascal Genschik, and Xinnian Dong. “Proteasome-mediated turnover of the transcription coactivator NPR1 plays dual roles in regulating plant immunity.Cell 137, no. 5 (May 2009): 860–72. https://doi.org/10.1016/j.cell.2009.03.038.
Spoel SH, Mou Z, Tada Y, Spivey NW, Genschik P, Dong X. Proteasome-mediated turnover of the transcription coactivator NPR1 plays dual roles in regulating plant immunity. Cell. 2009 May;137(5):860–72.
Spoel, Steven H., et al. “Proteasome-mediated turnover of the transcription coactivator NPR1 plays dual roles in regulating plant immunity.Cell, vol. 137, no. 5, May 2009, pp. 860–72. Epmc, doi:10.1016/j.cell.2009.03.038.
Spoel SH, Mou Z, Tada Y, Spivey NW, Genschik P, Dong X. Proteasome-mediated turnover of the transcription coactivator NPR1 plays dual roles in regulating plant immunity. Cell. 2009 May;137(5):860–872.
Journal cover image

Published In

Cell

DOI

EISSN

1097-4172

ISSN

0092-8674

Publication Date

May 2009

Volume

137

Issue

5

Start / End Page

860 / 872

Related Subject Headings

  • Transcription, Genetic
  • Proteasome Endopeptidase Complex
  • Phosphorylation
  • Immunity, Innate
  • Gene Expression Regulation, Plant
  • Developmental Biology
  • Cullin Proteins
  • Cell Nucleus
  • Carrier Proteins
  • Arabidopsis Proteins