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Modulation of nitric oxide production in human macrophages by apolipoprotein-E and amyloid-beta peptide.

Publication ,  Journal Article
Vitek, MP; Snell, J; Dawson, H; Colton, CA
Published in: Biochem Biophys Res Commun
November 17, 1997

Induction of oxidative stress has been implicated as a causative factor in chronic neurodegenerative diseases such as Alzheimer's disease. Apolipoprotein-E (apoE) and amyloid-beta peptide (A beta) have been reported to alter the redox state of the brain. Using human monocyte-derived macrophages as a model of brain microglia, physiological levels of apolipoprotein-E were found to stimulate nitric oxide (NO) production in polyinosinic:polycytidylic acid (poly I:C) primed cells. ApoE treatment released 68% more NO than cells treated with poly I:C alone and almost threefold more NO than unprimed cells. In contrast to mouse microglia, human cells failed to generate NO in response to A beta peptides, with or without poly I:C treatments. Furthermore, the combination of A beta plus apoE inhibited the increase in NO production induced by apoE. Since Alzheimer's is strongly associated with the presence of an APOE4 allele, our study predicts a mechanism where apoE and A beta regulate nitric oxide production in human brain.

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Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

November 17, 1997

Volume

240

Issue

2

Start / End Page

391 / 394

Location

United States

Related Subject Headings

  • Recombinant Proteins
  • Poly I-C
  • Nitric Oxide
  • Monocytes
  • Mice
  • Macrophages
  • Macrophage Colony-Stimulating Factor
  • Interferon-gamma
  • Humans
  • Cells, Cultured
 

Citation

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Vitek, M. P., Snell, J., Dawson, H., & Colton, C. A. (1997). Modulation of nitric oxide production in human macrophages by apolipoprotein-E and amyloid-beta peptide. Biochem Biophys Res Commun, 240(2), 391–394. https://doi.org/10.1006/bbrc.1997.7408
Vitek, M. P., J. Snell, H. Dawson, and C. A. Colton. “Modulation of nitric oxide production in human macrophages by apolipoprotein-E and amyloid-beta peptide.Biochem Biophys Res Commun 240, no. 2 (November 17, 1997): 391–94. https://doi.org/10.1006/bbrc.1997.7408.
Vitek MP, Snell J, Dawson H, Colton CA. Modulation of nitric oxide production in human macrophages by apolipoprotein-E and amyloid-beta peptide. Biochem Biophys Res Commun. 1997 Nov 17;240(2):391–4.
Vitek, M. P., et al. “Modulation of nitric oxide production in human macrophages by apolipoprotein-E and amyloid-beta peptide.Biochem Biophys Res Commun, vol. 240, no. 2, Nov. 1997, pp. 391–94. Pubmed, doi:10.1006/bbrc.1997.7408.
Vitek MP, Snell J, Dawson H, Colton CA. Modulation of nitric oxide production in human macrophages by apolipoprotein-E and amyloid-beta peptide. Biochem Biophys Res Commun. 1997 Nov 17;240(2):391–394.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

November 17, 1997

Volume

240

Issue

2

Start / End Page

391 / 394

Location

United States

Related Subject Headings

  • Recombinant Proteins
  • Poly I-C
  • Nitric Oxide
  • Monocytes
  • Mice
  • Macrophages
  • Macrophage Colony-Stimulating Factor
  • Interferon-gamma
  • Humans
  • Cells, Cultured