Skip to main content

An auxin gradient and maximum in the Arabidopsis root apex shown by high-resolution cell-specific analysis of IAA distribution and synthesis.

Publication ,  Journal Article
Petersson, SV; Johansson, AI; Kowalczyk, M; Makoveychuk, A; Wang, JY; Moritz, T; Grebe, M; Benfey, PN; Sandberg, G; Ljung, K
Published in: The Plant cell
June 2009

Local concentration gradients of the plant growth regulator auxin (indole-3-acetic acid [IAA]) are thought to instruct the positioning of organ primordia and stem cell niches and to direct cell division, expansion, and differentiation. High-resolution measurements of endogenous IAA concentrations in support of the gradient hypothesis are required to substantiate this hypothesis. Here, we introduce fluorescence-activated cell sorting of green fluorescent protein-marked cell types combined with highly sensitive mass spectrometry methods as a novel means for analyses of IAA distribution and metabolism at cellular resolution. Our results reveal the presence of IAA concentration gradients within the Arabidopsis thaliana root tip with a distinct maximum in the organizing quiescent center of the root apex. We also demonstrate that the root apex provides an important source of IAA and that cells of all types display a high synthesis capacity, suggesting a substantial contribution of local biosynthesis to auxin homeostasis in the root tip. Our results indicate that local biosynthesis and polar transport combine to produce auxin gradients and maxima in the root tip.

Altmetric Attention Stats
Dimensions Citation Stats

Published In

The Plant cell

DOI

EISSN

1532-298X

ISSN

1040-4651

Publication Date

June 2009

Volume

21

Issue

6

Start / End Page

1659 / 1668

Related Subject Headings

  • Protoplasts
  • Plant Roots
  • Plant Biology & Botany
  • Mass Spectrometry
  • Indoleacetic Acids
  • Homeostasis
  • Green Fluorescent Proteins
  • Flow Cytometry
  • Cell Size
  • Arabidopsis
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Petersson, S. V., Johansson, A. I., Kowalczyk, M., Makoveychuk, A., Wang, J. Y., Moritz, T., … Ljung, K. (2009). An auxin gradient and maximum in the Arabidopsis root apex shown by high-resolution cell-specific analysis of IAA distribution and synthesis. The Plant Cell, 21(6), 1659–1668. https://doi.org/10.1105/tpc.109.066480
Petersson, Sara V., Annika I. Johansson, Mariusz Kowalczyk, Alexander Makoveychuk, Jean Y. Wang, Thomas Moritz, Markus Grebe, Philip N. Benfey, Göran Sandberg, and Karin Ljung. “An auxin gradient and maximum in the Arabidopsis root apex shown by high-resolution cell-specific analysis of IAA distribution and synthesis.The Plant Cell 21, no. 6 (June 2009): 1659–68. https://doi.org/10.1105/tpc.109.066480.
Petersson SV, Johansson AI, Kowalczyk M, Makoveychuk A, Wang JY, Moritz T, et al. An auxin gradient and maximum in the Arabidopsis root apex shown by high-resolution cell-specific analysis of IAA distribution and synthesis. The Plant cell. 2009 Jun;21(6):1659–68.
Petersson, Sara V., et al. “An auxin gradient and maximum in the Arabidopsis root apex shown by high-resolution cell-specific analysis of IAA distribution and synthesis.The Plant Cell, vol. 21, no. 6, June 2009, pp. 1659–68. Epmc, doi:10.1105/tpc.109.066480.
Petersson SV, Johansson AI, Kowalczyk M, Makoveychuk A, Wang JY, Moritz T, Grebe M, Benfey PN, Sandberg G, Ljung K. An auxin gradient and maximum in the Arabidopsis root apex shown by high-resolution cell-specific analysis of IAA distribution and synthesis. The Plant cell. 2009 Jun;21(6):1659–1668.

Published In

The Plant cell

DOI

EISSN

1532-298X

ISSN

1040-4651

Publication Date

June 2009

Volume

21

Issue

6

Start / End Page

1659 / 1668

Related Subject Headings

  • Protoplasts
  • Plant Roots
  • Plant Biology & Botany
  • Mass Spectrometry
  • Indoleacetic Acids
  • Homeostasis
  • Green Fluorescent Proteins
  • Flow Cytometry
  • Cell Size
  • Arabidopsis