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Rapid global expansion of the fungal disease chytridiomycosis into declining and healthy amphibian populations.

Publication ,  Journal Article
James, TY; Litvintseva, AP; Vilgalys, R; Morgan, JA; Taylor, JW; Fisher, MC; Berger, L; Weldon, C; du Preez, L; Longcore, JE
Published in: PLoS Pathog
May 2009

The fungal disease chytridiomycosis, caused by Batrachochytrium dendrobatidis, is enigmatic because it occurs globally in both declining and apparently healthy (non-declining) amphibian populations. This distribution has fueled debate concerning whether, in sites where it has recently been found, the pathogen was introduced or is endemic. In this study, we addressed the molecular population genetics of a global collection of fungal strains from both declining and healthy amphibian populations using DNA sequence variation from 17 nuclear loci and a large fragment from the mitochondrial genome. We found a low rate of DNA polymorphism, with only two sequence alleles detected at each locus, but a high diversity of diploid genotypes. Half of the loci displayed an excess of heterozygous genotypes, consistent with a primarily clonal mode of reproduction. Despite the absence of obvious sex, genotypic diversity was high (44 unique genotypes out of 59 strains). We provide evidence that the observed genotypic variation can be generated by loss of heterozygosity through mitotic recombination. One strain isolated from a bullfrog possessed as much allelic diversity as the entire global sample, suggesting the current epidemic can be traced back to the outbreak of a single clonal lineage. These data are consistent with the current chytridiomycosis epidemic resulting from a novel pathogen undergoing a rapid and recent range expansion. The widespread occurrence of the same lineage in both healthy and declining populations suggests that the outcome of the disease is contingent on environmental factors and host resistance.

Duke Scholars

Published In

PLoS Pathog

Publication Date

May 2009

Volume

5

Issue

5

Start / End Page

e1000458

Related Subject Headings

  • Virology
  • Recombination, Genetic
  • Rana catesbeiana
  • Polymorphism, Genetic
  • Mycoses
  • Loss of Heterozygosity
  • Genotype
  • Genome, Mitochondrial
  • Disease Outbreaks
  • DNA, Viral
 

Citation

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James, T. Y., Litvintseva, A. P., Vilgalys, R., Morgan, J. A., Taylor, J. W., Fisher, M. C., … Longcore, J. E. (2009). Rapid global expansion of the fungal disease chytridiomycosis into declining and healthy amphibian populations. PLoS Pathog, 5(5), e1000458.
James, T. Y., A. P. Litvintseva, R. Vilgalys, J. A. Morgan, J. W. Taylor, M. C. Fisher, L. Berger, C. Weldon, L. du Preez, and J. E. Longcore. “Rapid global expansion of the fungal disease chytridiomycosis into declining and healthy amphibian populations.PLoS Pathog 5, no. 5 (May 2009): e1000458.
James TY, Litvintseva AP, Vilgalys R, Morgan JA, Taylor JW, Fisher MC, et al. Rapid global expansion of the fungal disease chytridiomycosis into declining and healthy amphibian populations. PLoS Pathog. 2009 May;5(5):e1000458.
James, T. Y., et al. “Rapid global expansion of the fungal disease chytridiomycosis into declining and healthy amphibian populations.PLoS Pathog, vol. 5, no. 5, May 2009, p. e1000458.
James TY, Litvintseva AP, Vilgalys R, Morgan JA, Taylor JW, Fisher MC, Berger L, Weldon C, du Preez L, Longcore JE. Rapid global expansion of the fungal disease chytridiomycosis into declining and healthy amphibian populations. PLoS Pathog. 2009 May;5(5):e1000458.

Published In

PLoS Pathog

Publication Date

May 2009

Volume

5

Issue

5

Start / End Page

e1000458

Related Subject Headings

  • Virology
  • Recombination, Genetic
  • Rana catesbeiana
  • Polymorphism, Genetic
  • Mycoses
  • Loss of Heterozygosity
  • Genotype
  • Genome, Mitochondrial
  • Disease Outbreaks
  • DNA, Viral