Identification of calcium oxalate crystals using alizarin red S stain.
Publication
, Journal Article
Proia, AD; Brinn, NT
Published in: Arch Pathol Lab Med
February 1985
Calcium oxalate crystals stain with alizarin red S at a pH of 7.0 but not at a pH of 4.2. In contrast, calcium phosphate and calcium carbonate stain at a pH of both 7.0 and 4.2. This difference allows presumptive identification of calcium oxalate deposits. The identity of calcium oxalate can then be confirmed by its insolubility in 2M acetic acid, since both calcium carbonate and calcium phosphate are soluble. We have applied this procedure for several years and have found it to be a rapid, reliable, and technically simple procedure for distinguishing calcium oxalate from other calcium deposits.
Duke Scholars
Published In
Arch Pathol Lab Med
ISSN
0003-9985
Publication Date
February 1985
Volume
109
Issue
2
Start / End Page
186 / 189
Location
United States
Related Subject Headings
- Staining and Labeling
- Solubility
- Rats, Inbred Strains
- Rats
- Pathology
- Oxalic Acid
- Oxalates
- Kidney Failure, Chronic
- Kidney
- Hydrogen-Ion Concentration
Citation
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ICMJE
MLA
NLM
Proia, A. D., & Brinn, N. T. (1985). Identification of calcium oxalate crystals using alizarin red S stain. Arch Pathol Lab Med, 109(2), 186–189.
Proia, A. D., and N. T. Brinn. “Identification of calcium oxalate crystals using alizarin red S stain.” Arch Pathol Lab Med 109, no. 2 (February 1985): 186–89.
Proia AD, Brinn NT. Identification of calcium oxalate crystals using alizarin red S stain. Arch Pathol Lab Med. 1985 Feb;109(2):186–9.
Proia, A. D., and N. T. Brinn. “Identification of calcium oxalate crystals using alizarin red S stain.” Arch Pathol Lab Med, vol. 109, no. 2, Feb. 1985, pp. 186–89.
Proia AD, Brinn NT. Identification of calcium oxalate crystals using alizarin red S stain. Arch Pathol Lab Med. 1985 Feb;109(2):186–189.
Published In
Arch Pathol Lab Med
ISSN
0003-9985
Publication Date
February 1985
Volume
109
Issue
2
Start / End Page
186 / 189
Location
United States
Related Subject Headings
- Staining and Labeling
- Solubility
- Rats, Inbred Strains
- Rats
- Pathology
- Oxalic Acid
- Oxalates
- Kidney Failure, Chronic
- Kidney
- Hydrogen-Ion Concentration