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Interferon gamma-inducible protein-10 (IP-10) and eotaxin as biomarkers in age-related macular degeneration.

Publication ,  Journal Article
Mo, FM; Proia, AD; Johnson, WH; Cyr, D; Lashkari, K
Published in: Invest Ophthalmol Vis Sci
August 2010

PURPOSE: To analyze serum cytokine levels in subjects with different stages of AMD and to study the expression of salient cytokines in postmortem eyes with AMD. METHODS: A suspension array system was used to analyze sera (n = 18 to 20/group) from control subjects and those with early AMD (AREDS stage 1), intermediate dry AMD (AREDS stage 3), advanced AMD with geographic atrophy (GA), or neovascular AMD (CNV). Postmortem eyes with AMD or control eyes were examined immunohistochemically for expression of IP-10 and eotaxin (n = 4 to 8/group). RESULTS: Serum eotaxin and IP-10 levels were significantly elevated in all stages of AMD, except for eotaxin levels in neovascular AMD (P < 0.07). The peak of serum IP-10 concentration was at intermediate dry AMD. In donor eyes, IP-10 and eotaxin expressions were increased in the RPE of eyes with early AMD, GA, and CNV. Eotaxin accumulated within the layer of basal linear/laminar deposits in all stages of AMD, while IP-10 was mainly in eyes with GA and CNV. IP-10 was abundant in the connective tissue matrix associated with CNV, and eotaxin was usually present but more focally and with less intense staining. Both IP-10 and eotaxin were expressed by neovascular endothelial cells. Both IP-10 and eotaxin were expressed in the neurosensory retina, but there was no detectable difference in staining between eyes with or without AMD. CONCLUSIONS: IP-10 and eotaxin may be early biomarkers in AMD. The authors hypothesize that the relative balance between levels of IP-10 and eotaxin is critical in regulating the neovascular response.

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Published In

Invest Ophthalmol Vis Sci

DOI

EISSN

1552-5783

Publication Date

August 2010

Volume

51

Issue

8

Start / End Page

4226 / 4236

Location

United States

Related Subject Headings

  • Retinal Pigment Epithelium
  • Ophthalmology & Optometry
  • Male
  • Macular Degeneration
  • Immunoenzyme Techniques
  • Humans
  • Geographic Atrophy
  • Female
  • Choroidal Neovascularization
  • Chemokine CXCL10
 

Citation

APA
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ICMJE
MLA
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Mo, F. M., Proia, A. D., Johnson, W. H., Cyr, D., & Lashkari, K. (2010). Interferon gamma-inducible protein-10 (IP-10) and eotaxin as biomarkers in age-related macular degeneration. Invest Ophthalmol Vis Sci, 51(8), 4226–4236. https://doi.org/10.1167/iovs.09-3910
Mo, Fong Ming, Alan D. Proia, Walter H. Johnson, Desiree Cyr, and Kameran Lashkari. “Interferon gamma-inducible protein-10 (IP-10) and eotaxin as biomarkers in age-related macular degeneration.Invest Ophthalmol Vis Sci 51, no. 8 (August 2010): 4226–36. https://doi.org/10.1167/iovs.09-3910.
Mo FM, Proia AD, Johnson WH, Cyr D, Lashkari K. Interferon gamma-inducible protein-10 (IP-10) and eotaxin as biomarkers in age-related macular degeneration. Invest Ophthalmol Vis Sci. 2010 Aug;51(8):4226–36.
Mo, Fong Ming, et al. “Interferon gamma-inducible protein-10 (IP-10) and eotaxin as biomarkers in age-related macular degeneration.Invest Ophthalmol Vis Sci, vol. 51, no. 8, Aug. 2010, pp. 4226–36. Pubmed, doi:10.1167/iovs.09-3910.
Mo FM, Proia AD, Johnson WH, Cyr D, Lashkari K. Interferon gamma-inducible protein-10 (IP-10) and eotaxin as biomarkers in age-related macular degeneration. Invest Ophthalmol Vis Sci. 2010 Aug;51(8):4226–4236.

Published In

Invest Ophthalmol Vis Sci

DOI

EISSN

1552-5783

Publication Date

August 2010

Volume

51

Issue

8

Start / End Page

4226 / 4236

Location

United States

Related Subject Headings

  • Retinal Pigment Epithelium
  • Ophthalmology & Optometry
  • Male
  • Macular Degeneration
  • Immunoenzyme Techniques
  • Humans
  • Geographic Atrophy
  • Female
  • Choroidal Neovascularization
  • Chemokine CXCL10