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Calcium-dependent regulation of cholecystokinin secretion and potassium currents in STC-1 cells.

Publication ,  Journal Article
Mangel, AW; Snow, ND; Misukonis, MA; Basavappa, S; Middleton, JP; Fitz, JG; Liddle, RA
Published in: Am J Physiol
June 1993

Secretory and electrophysiological properties of STC-1 cells, a cholecystokinin-secreting cell line, were examined with a radioimmunoassay and patch-clamp recording techniques. Stimulation of cholecystokinin secretion was seen after exposure to agents anticipated to increase the level of intracellular calcium, including thapsigargin (8 microM), bombesin (50 nM), potassium-induced depolarization (50 mM), or after blockade of potassium channels with barium chloride (2 mM). The secretory effects of these agents were blocked by pretreatment with the calcium channel blocker diltiazem (1 microM). Whole cell patch-clamp recordings showed a hyperpolarizing shift in reversal potential after exposure to either thapsigargin (8 microM) or bombesin (50 nM) from a control value of -27 +/- 3 to -57 +/- 7 or -48 +/- 6 mV, respectively. This shift was in the direction of the reversal potential for potassium and was blocked by barium chloride (5 mM). Single-channel recordings from cell-attached membrane patches showed an inwardly rectifying potassium channel with channel open probability modulated by bombesin. These results indicate that in STC-1 cells a potassium current is increased by agents that stimulate CCK secretion, presumably by increasing the level of cytosolic calcium. STC-1 cells may serve as a model system to study the electrophysiological and secretory mechanisms involved in the release of cholecystokinin.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

June 1993

Volume

264

Issue

6 Pt 1

Start / End Page

G1031 / G1036

Location

United States

Related Subject Headings

  • Potassium
  • Mice, Transgenic
  • Mice
  • Electric Conductivity
  • Cholecystokinin
  • Cell Line
  • Cardiovascular System & Hematology
  • Calcium
  • Animals
 

Citation

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ICMJE
MLA
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Mangel, A. W., Snow, N. D., Misukonis, M. A., Basavappa, S., Middleton, J. P., Fitz, J. G., & Liddle, R. A. (1993). Calcium-dependent regulation of cholecystokinin secretion and potassium currents in STC-1 cells. Am J Physiol, 264(6 Pt 1), G1031–G1036. https://doi.org/10.1152/ajpgi.1993.264.6.G1031
Mangel, A. W., N. D. Snow, M. A. Misukonis, S. Basavappa, J. P. Middleton, J. G. Fitz, and R. A. Liddle. “Calcium-dependent regulation of cholecystokinin secretion and potassium currents in STC-1 cells.Am J Physiol 264, no. 6 Pt 1 (June 1993): G1031–36. https://doi.org/10.1152/ajpgi.1993.264.6.G1031.
Mangel AW, Snow ND, Misukonis MA, Basavappa S, Middleton JP, Fitz JG, et al. Calcium-dependent regulation of cholecystokinin secretion and potassium currents in STC-1 cells. Am J Physiol. 1993 Jun;264(6 Pt 1):G1031–6.
Mangel, A. W., et al. “Calcium-dependent regulation of cholecystokinin secretion and potassium currents in STC-1 cells.Am J Physiol, vol. 264, no. 6 Pt 1, June 1993, pp. G1031–36. Pubmed, doi:10.1152/ajpgi.1993.264.6.G1031.
Mangel AW, Snow ND, Misukonis MA, Basavappa S, Middleton JP, Fitz JG, Liddle RA. Calcium-dependent regulation of cholecystokinin secretion and potassium currents in STC-1 cells. Am J Physiol. 1993 Jun;264(6 Pt 1):G1031–G1036.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

June 1993

Volume

264

Issue

6 Pt 1

Start / End Page

G1031 / G1036

Location

United States

Related Subject Headings

  • Potassium
  • Mice, Transgenic
  • Mice
  • Electric Conductivity
  • Cholecystokinin
  • Cell Line
  • Cardiovascular System & Hematology
  • Calcium
  • Animals