![Journal cover image](https://secure.syndetics.com/index.aspx?isbn=/lc.gif&issn=1095-9572&client=dukeuniv)
Microscopic diffusion tensor imaging of the mouse brain.
Diffusion tensor imaging (DTI) data at 43 mum isotropic resolution has been acquired on the intact adult mouse brain in 28-h scan time by using a streamlined protocol, including specimen fixation and staining, image acquisition, reconstruction, post-processing, and distribution. An intermediate registration of each component image is required to achieve the desired microscopic resolution. Multiple parameters have been derived, including fractional anisotropy, axial and radial diffusivity, and a color-coded orientation map of the primary eigenvector. Each DTI dataset was mapped to a common reference space to facilitate future standardized analysis. Fiber tracking has also been demonstrated, providing 3D connection information. This protocol to acquire high-resolution DTI data in a robust and repeatable fashion will serve as a foundation to quantitatively study mouse brain integrity and white matter architecture, at what we believe to be the highest spatial resolution yet attained.
Duke Scholars
Altmetric Attention Stats
Dimensions Citation Stats
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Tissue Fixation
- Staining and Labeling
- Neurology & Neurosurgery
- Mice
- Image Interpretation, Computer-Assisted
- Diffusion Tensor Imaging
- Brain Mapping
- Brain
- Anisotropy
- Animals
Citation
![Journal cover image](https://secure.syndetics.com/index.aspx?isbn=/lc.gif&issn=1095-9572&client=dukeuniv)
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Tissue Fixation
- Staining and Labeling
- Neurology & Neurosurgery
- Mice
- Image Interpretation, Computer-Assisted
- Diffusion Tensor Imaging
- Brain Mapping
- Brain
- Anisotropy
- Animals