Skip to main content
Journal cover image

Endogenous S-nitrosothiols protect against myocardial injury.

Publication ,  Journal Article
Lima, B; Lam, GKW; Xie, L; Diesen, DL; Villamizar, N; Nienaber, J; Messina, E; Bowles, D; Kontos, CD; Hare, JM; Stamler, JS; Rockman, HA
Published in: Proc Natl Acad Sci U S A
April 14, 2009

Despite substantial evidence that nitric oxide (NO) and/or endogenous S-nitrosothiols (SNOs) exert protective effects in a variety of cardiovascular diseases, the molecular details are largely unknown. Here we show that following left coronary artery ligation, mice with a targeted deletion of the S-nitrosoglutathione reductase gene (GSNOR(-/-)) have reduced myocardial infarct size, preserved ventricular systolic and diastolic function, and maintained tissue oxygenation. These profound physiological effects are associated with increases in myocardial capillary density and S-nitrosylation of the transcription factor hypoxia inducible factor-1alpha (HIF-1alpha) under normoxic conditions. We further show that S-nitrosylated HIF-1alpha binds to the vascular endothelial growth factor (VEGF) gene, thus identifying a role for GSNO in angiogenesis and myocardial protection. These results suggest innovative approaches to modulate angiogenesis and preserve cardiac function.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

April 14, 2009

Volume

106

Issue

15

Start / End Page

6297 / 6302

Location

United States

Related Subject Headings

  • Vascular Endothelial Growth Factor A
  • Transcription, Genetic
  • Time Factors
  • S-Nitrosothiols
  • Protein Binding
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Hypoxia-Inducible Factor 1
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lima, B., Lam, G. K. W., Xie, L., Diesen, D. L., Villamizar, N., Nienaber, J., … Rockman, H. A. (2009). Endogenous S-nitrosothiols protect against myocardial injury. Proc Natl Acad Sci U S A, 106(15), 6297–6302. https://doi.org/10.1073/pnas.0901043106
Lima, Brian, Gregory K. W. Lam, Liang Xie, Diana L. Diesen, Nestor Villamizar, Jeffrey Nienaber, Emily Messina, et al. “Endogenous S-nitrosothiols protect against myocardial injury.Proc Natl Acad Sci U S A 106, no. 15 (April 14, 2009): 6297–6302. https://doi.org/10.1073/pnas.0901043106.
Lima B, Lam GKW, Xie L, Diesen DL, Villamizar N, Nienaber J, et al. Endogenous S-nitrosothiols protect against myocardial injury. Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6297–302.
Lima, Brian, et al. “Endogenous S-nitrosothiols protect against myocardial injury.Proc Natl Acad Sci U S A, vol. 106, no. 15, Apr. 2009, pp. 6297–302. Pubmed, doi:10.1073/pnas.0901043106.
Lima B, Lam GKW, Xie L, Diesen DL, Villamizar N, Nienaber J, Messina E, Bowles D, Kontos CD, Hare JM, Stamler JS, Rockman HA. Endogenous S-nitrosothiols protect against myocardial injury. Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6297–6302.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

April 14, 2009

Volume

106

Issue

15

Start / End Page

6297 / 6302

Location

United States

Related Subject Headings

  • Vascular Endothelial Growth Factor A
  • Transcription, Genetic
  • Time Factors
  • S-Nitrosothiols
  • Protein Binding
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Hypoxia-Inducible Factor 1