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Embryonic mouse lung epithelial progenitor cells co-express immunohistochemical markers of diverse mature cell lineages.

Publication ,  Journal Article
Wuenschell, CW; Sunday, ME; Singh, G; Minoo, P; Slavkin, HC; Warburton, D
Published in: J Histochem Cytochem
February 1996

Developmental expression of marker genes representative of different mature cell types can be used to study differentiation of cell lineages. We used immunohistochemistry to study expression in developing mouse lung of calcitonin gene-related peptide (CGRP), Clara cell 10-KD protein (CC10), and surfactant protein-A (SP-A), markers that are differentially expressed in neuroendocrine cells, Clara cells, and Type II alveolar cells. Two distinct developmental phases were revealed. The earlier phase (embryonic days 13-15; E13-E15) was characterized by CGRP, CC10, and SP-A immunostaining in all epithelial cells of the distal airways, with the three patterns being virtually identical in adjacent sections. The later phase (E16-E18) was characterized by emergence of staining of the differentiated cell types. These expression patterns were recapitulated in serumless organ culture, demonstrating that information necessary to generate both phases of gene expression is present within the lung analage by E11. We conclude that CGRP, CC10, and SP-A are co-expressed in most or all cells of the distal lung epithelium at E13-E15 and later become restricted to different cell lineages. This transient expression in progenitor cells of gene products characteristic of diverse differentiated cell types may reflect an underlying mechanism of gene regulation.

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Published In

J Histochem Cytochem

DOI

ISSN

0022-1554

Publication Date

February 1996

Volume

44

Issue

2

Start / End Page

113 / 123

Location

United States

Related Subject Headings

  • Uteroglobin
  • Stem Cells
  • Pulmonary Surfactants
  • Pulmonary Surfactant-Associated Proteins
  • Pulmonary Surfactant-Associated Protein A
  • Proteolipids
  • Proteins
  • Protein Biosynthesis
  • Pregnancy
  • Organ Culture Techniques
 

Citation

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Wuenschell, C. W., Sunday, M. E., Singh, G., Minoo, P., Slavkin, H. C., & Warburton, D. (1996). Embryonic mouse lung epithelial progenitor cells co-express immunohistochemical markers of diverse mature cell lineages. J Histochem Cytochem, 44(2), 113–123. https://doi.org/10.1177/44.2.8609367
Wuenschell, C. W., M. E. Sunday, G. Singh, P. Minoo, H. C. Slavkin, and D. Warburton. “Embryonic mouse lung epithelial progenitor cells co-express immunohistochemical markers of diverse mature cell lineages.J Histochem Cytochem 44, no. 2 (February 1996): 113–23. https://doi.org/10.1177/44.2.8609367.
Wuenschell CW, Sunday ME, Singh G, Minoo P, Slavkin HC, Warburton D. Embryonic mouse lung epithelial progenitor cells co-express immunohistochemical markers of diverse mature cell lineages. J Histochem Cytochem. 1996 Feb;44(2):113–23.
Wuenschell, C. W., et al. “Embryonic mouse lung epithelial progenitor cells co-express immunohistochemical markers of diverse mature cell lineages.J Histochem Cytochem, vol. 44, no. 2, Feb. 1996, pp. 113–23. Pubmed, doi:10.1177/44.2.8609367.
Wuenschell CW, Sunday ME, Singh G, Minoo P, Slavkin HC, Warburton D. Embryonic mouse lung epithelial progenitor cells co-express immunohistochemical markers of diverse mature cell lineages. J Histochem Cytochem. 1996 Feb;44(2):113–123.
Journal cover image

Published In

J Histochem Cytochem

DOI

ISSN

0022-1554

Publication Date

February 1996

Volume

44

Issue

2

Start / End Page

113 / 123

Location

United States

Related Subject Headings

  • Uteroglobin
  • Stem Cells
  • Pulmonary Surfactants
  • Pulmonary Surfactant-Associated Proteins
  • Pulmonary Surfactant-Associated Protein A
  • Proteolipids
  • Proteins
  • Protein Biosynthesis
  • Pregnancy
  • Organ Culture Techniques