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Regulation of the G protein Galphai2 by growth and development in fetal airway epithelium.

Publication ,  Journal Article
Kinane, TB; Komatsuzaki, K; Aleixo, MD; Sunday, ME; Ercolani, L
Published in: Am J Respir Cell Mol Biol
January 1999

Heterotrimeric guanine nucleotide-binding (G) proteins transduce a wide variety of receptor-mediated signals to effectors that are involved in numerous cellular functions, including cell proliferation and differentiation. Thrombin and bombesin/gastrin-releasing peptide mediate their effects via G protein-coupled receptors to regulate lung growth and development. The growth responses of these ligands are likely to be mediated via the Gi subfamily of G proteins, specifically via Galphai2. We hypothesized that Galphai2 is expressed in the lung during ontogeny in a growth-dependent manner, and that Galphai2 regulates cell growth. We demonstrate that Galphai2 is present in the developing lung of Sprague-Dawley rats, and that its expression is enhanced between embryonic Day 19 and postnatal Day 2. The strongest expression occurs in the fetal airway epithelium, and this expression in fetal airway cells is growth-dependent. Galphai2 is localized to the plasma membrane, a location consistent with interaction with growth factor receptors. Inhibition of Gi-family signal transduction by pertussis toxin (10 ng/ml) inhibits DNA synthesis in embryonic Day 19 in fetal airway epithelium. Galphai2 is likely to be a key mediator of growth signals in the developing lung.

Duke Scholars

Published In

Am J Respir Cell Mol Biol

DOI

ISSN

1044-1549

Publication Date

January 1999

Volume

20

Issue

1

Start / End Page

35 / 42

Location

United States

Related Subject Headings

  • Virulence Factors, Bordetella
  • Tissue Distribution
  • Signal Transduction
  • Respiratory System
  • Rats, Sprague-Dawley
  • Rats
  • Pregnancy
  • Pertussis Toxin
  • Lung
  • Golgi Apparatus
 

Citation

APA
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ICMJE
MLA
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Kinane, T. B., Komatsuzaki, K., Aleixo, M. D., Sunday, M. E., & Ercolani, L. (1999). Regulation of the G protein Galphai2 by growth and development in fetal airway epithelium. Am J Respir Cell Mol Biol, 20(1), 35–42. https://doi.org/10.1165/ajrcmb.20.1.3297
Kinane, T. B., K. Komatsuzaki, M. D. Aleixo, M. E. Sunday, and L. Ercolani. “Regulation of the G protein Galphai2 by growth and development in fetal airway epithelium.Am J Respir Cell Mol Biol 20, no. 1 (January 1999): 35–42. https://doi.org/10.1165/ajrcmb.20.1.3297.
Kinane TB, Komatsuzaki K, Aleixo MD, Sunday ME, Ercolani L. Regulation of the G protein Galphai2 by growth and development in fetal airway epithelium. Am J Respir Cell Mol Biol. 1999 Jan;20(1):35–42.
Kinane, T. B., et al. “Regulation of the G protein Galphai2 by growth and development in fetal airway epithelium.Am J Respir Cell Mol Biol, vol. 20, no. 1, Jan. 1999, pp. 35–42. Pubmed, doi:10.1165/ajrcmb.20.1.3297.
Kinane TB, Komatsuzaki K, Aleixo MD, Sunday ME, Ercolani L. Regulation of the G protein Galphai2 by growth and development in fetal airway epithelium. Am J Respir Cell Mol Biol. 1999 Jan;20(1):35–42.

Published In

Am J Respir Cell Mol Biol

DOI

ISSN

1044-1549

Publication Date

January 1999

Volume

20

Issue

1

Start / End Page

35 / 42

Location

United States

Related Subject Headings

  • Virulence Factors, Bordetella
  • Tissue Distribution
  • Signal Transduction
  • Respiratory System
  • Rats, Sprague-Dawley
  • Rats
  • Pregnancy
  • Pertussis Toxin
  • Lung
  • Golgi Apparatus