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Control of basement membrane remodeling and epithelial branching morphogenesis in embryonic lung by Rho and cytoskeletal tension.

Publication ,  Journal Article
Moore, KA; Polte, T; Huang, S; Shi, B; Alsberg, E; Sunday, ME; Ingber, DE
Published in: Dev Dyn
February 2005

Local alterations in the mechanical compliance of the basement membrane that alter the level of isometric tension in the cell have been postulated to influence tissue morphogenesis. To explore whether cell tension contributes to tissue pattern formation in vivo, we modulated cytoskeletal force generation in embryonic mouse lung (embryonic days 12-14) rudiments using inhibitors of Rho-associated kinase (ROCK), myosin light chain kinase, myosin ATPase, and microfilament integrity, or a Rho stimulator (cytotoxic necrotizing factor-1). Tension inhibition resulted in loss of normal differentials in basement membrane thickness, inhibition of new terminal bud formation, and disorganization of epithelial growth patterns as well as disruption of capillary blood vessels. In contrast, increasing cell tension through Rho activation, as confirmed by quantitation of myosin light chain phosphorylation and immunohistocytochemical analysis of actin organization, accelerated lung branching and increase capillary elongation. These data suggest that changes in cytoskeletal tension mediated by Rho signaling through ROCK may play an important role in the establishment of the spatial differentials in cell growth and extracellular matrix remodeling that drive embryonic lung development.

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Published In

Dev Dyn

DOI

ISSN

1058-8388

Publication Date

February 2005

Volume

232

Issue

2

Start / End Page

268 / 281

Location

United States

Related Subject Headings

  • rho GTP-Binding Proteins
  • Time Factors
  • Signal Transduction
  • Pyridines
  • Phosphorylation
  • Myosins
  • Myosin Light Chains
  • Mice
  • Lung
  • Immunohistochemistry
 

Citation

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Moore, K. A., Polte, T., Huang, S., Shi, B., Alsberg, E., Sunday, M. E., & Ingber, D. E. (2005). Control of basement membrane remodeling and epithelial branching morphogenesis in embryonic lung by Rho and cytoskeletal tension. Dev Dyn, 232(2), 268–281. https://doi.org/10.1002/dvdy.20237
Moore, Kimberly A., Tom Polte, Sui Huang, Bin Shi, Eben Alsberg, Mary E. Sunday, and Donald E. Ingber. “Control of basement membrane remodeling and epithelial branching morphogenesis in embryonic lung by Rho and cytoskeletal tension.Dev Dyn 232, no. 2 (February 2005): 268–81. https://doi.org/10.1002/dvdy.20237.
Moore KA, Polte T, Huang S, Shi B, Alsberg E, Sunday ME, et al. Control of basement membrane remodeling and epithelial branching morphogenesis in embryonic lung by Rho and cytoskeletal tension. Dev Dyn. 2005 Feb;232(2):268–81.
Moore, Kimberly A., et al. “Control of basement membrane remodeling and epithelial branching morphogenesis in embryonic lung by Rho and cytoskeletal tension.Dev Dyn, vol. 232, no. 2, Feb. 2005, pp. 268–81. Pubmed, doi:10.1002/dvdy.20237.
Moore KA, Polte T, Huang S, Shi B, Alsberg E, Sunday ME, Ingber DE. Control of basement membrane remodeling and epithelial branching morphogenesis in embryonic lung by Rho and cytoskeletal tension. Dev Dyn. 2005 Feb;232(2):268–281.
Journal cover image

Published In

Dev Dyn

DOI

ISSN

1058-8388

Publication Date

February 2005

Volume

232

Issue

2

Start / End Page

268 / 281

Location

United States

Related Subject Headings

  • rho GTP-Binding Proteins
  • Time Factors
  • Signal Transduction
  • Pyridines
  • Phosphorylation
  • Myosins
  • Myosin Light Chains
  • Mice
  • Lung
  • Immunohistochemistry