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Three-dimensional imaging of whole rodent organs using optical computed and emission tomography.

Publication ,  Journal Article
Oldham, M; Sakhalkar, H; Wang, YM; Guo, P; Oliver, T; Bentley, R; Vujaskovic, Z; Dewhirst, M
Published in: J Biomed Opt
2007

We explore the potential of optical computed tomography (optical-CT) and optical emission computed tomography (optical-ECT) in a new area-whole organ imaging. The techniques are implemented on an in-house prototype benchtop system with improved image quality and the capacity to image larger samples (up to 3 cm) than previous systems based on stereo microscopes. Imaging performance tests confirm high geometrical accuracy, accurate relative measurement of linear attenuation coefficients, and the ability to image features at the 50-microm level. Optical labeling of organ microvasculature was achieved using two stains deposited via natural in vivo circulatory processes: a passive absorbing ink-based stain and an active fluorescin FITC-lectin conjugate. The lectin protein binds to the endothelial lining, and FITC fluorescense enables optical-ECT imaging. Three-dimensional (3-D) optical-CT images have been acquired of a normal rat heart and left lung and a mouse right lung showing exquisite detail of the functional vasculature and relative perfusion distribution. Coregistered optical-ECT images were also acquired of the mouse lung and kidney. Histological sections confirmed effective labeling of microvasculature throughout the organs. The advantages of optical-CT and optical-ECT include the potential for a unique combination of high resolution and high contrast and compatibility with a wide variety of optical probes, including gene expression labeling fluorescent reporter proteins.

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Published In

J Biomed Opt

DOI

ISSN

1083-3668

Publication Date

2007

Volume

12

Issue

1

Start / End Page

014009

Location

United States

Related Subject Headings

  • Viscera
  • Tomography, Optical
  • Tomography, Emission-Computed
  • Sensitivity and Specificity
  • Reproducibility of Results
  • Rats
  • Pilot Projects
  • Optics
  • Mice
  • Imaging, Three-Dimensional
 

Citation

APA
Chicago
ICMJE
MLA
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Oldham, M., Sakhalkar, H., Wang, Y. M., Guo, P., Oliver, T., Bentley, R., … Dewhirst, M. (2007). Three-dimensional imaging of whole rodent organs using optical computed and emission tomography. J Biomed Opt, 12(1), 014009. https://doi.org/10.1117/1.2709858
Oldham, Mark, Harshad Sakhalkar, Ying Min Wang, Pengyi Guo, Tim Oliver, Rex Bentley, Zeljko Vujaskovic, and Mark Dewhirst. “Three-dimensional imaging of whole rodent organs using optical computed and emission tomography.J Biomed Opt 12, no. 1 (2007): 014009. https://doi.org/10.1117/1.2709858.
Oldham M, Sakhalkar H, Wang YM, Guo P, Oliver T, Bentley R, et al. Three-dimensional imaging of whole rodent organs using optical computed and emission tomography. J Biomed Opt. 2007;12(1):014009.
Oldham, Mark, et al. “Three-dimensional imaging of whole rodent organs using optical computed and emission tomography.J Biomed Opt, vol. 12, no. 1, 2007, p. 014009. Pubmed, doi:10.1117/1.2709858.
Oldham M, Sakhalkar H, Wang YM, Guo P, Oliver T, Bentley R, Vujaskovic Z, Dewhirst M. Three-dimensional imaging of whole rodent organs using optical computed and emission tomography. J Biomed Opt. 2007;12(1):014009.

Published In

J Biomed Opt

DOI

ISSN

1083-3668

Publication Date

2007

Volume

12

Issue

1

Start / End Page

014009

Location

United States

Related Subject Headings

  • Viscera
  • Tomography, Optical
  • Tomography, Emission-Computed
  • Sensitivity and Specificity
  • Reproducibility of Results
  • Rats
  • Pilot Projects
  • Optics
  • Mice
  • Imaging, Three-Dimensional